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ProRule PRU00680


General rule information [?]

Accession PRU00680
Dates 18-DEC-2007 (Created)
19-NOV-2022 (Last updated, Version 12)
Data class Domain;
Predictors PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2
Name Glycosyl hydrolases family 22 (GH22) domain
Function Alpha-lactalbumin, a regulatory protein in milk, and lysozyme (EC 3.2.1.17) C (chicken type), which acts as a bacteriolytic enzyme, belong to a family of proteins with eight conserved cysteines that form four disulfide bonds. Alpha-lactalbumin lacks the enzymatic activity and the corresponding Glu and Asp residues. Alpha-lactalbumin binds a calcium ion, while most lysozyme C do not bind calcium.
Scope(s) Eukaryota
Metazoa
Example(s) Q659U0 (LYSC_LEPWE);

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + AltName: Full=lysozyme C;
                 EC=3.2.1.17;
AltName: Full=1,4-beta-N-acetylmuramidase C;

Comments [?]

FUNCTIONLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
CATALYTIC ACTIVITY Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
end case
SIMILARITYBelongs to the glycosyl hydrolase 22 family.

Keywords [?]

case <FTGroup:1>
Antimicrobial
Bacteriolytic enzyme
Glycosidase
Hydrolase
end case
case <Feature:PS51348:85=D> and <Feature:PS51348:90-91=D-D>
Calcium
end case
case <FTTag:disulf>
Disulfide bond
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0003824; Molecular function:catalytic activity
GO:0019835; Biological process:cytolysis
GO:0042742; Biological process:defense response to bacterium
GO:0016798; Molecular function:hydrolase activity, acting on glycosyl bonds
GO:0008152; Biological process:metabolic process
GO:0016787; Molecular function:hydrolase activity
end case
case <Feature:PS51348:85=D> and <Feature:PS51348:90-91=D-D>
GO:0005509; Molecular function:calcium ion binding
end case

Cross-references [?]

PROSITE PS00128; GLYCOSYL_HYDROL_F22_1; 1;

Features [?]

From: PS51348
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="C-type lysozyme #"
ACT_SITE 35 35 E 1
ACT_SITE 52 52 D 1
case <Feature:PS51348:85=D> and <Feature:PS51348:90-91=D-D>
BINDING 82 82 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="#1"		 binds [HCUEDKQNMYWSTRX]
BINDING 85 85 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="#1"		 binds [HCUEDKQNMYWSTRX]
BINDING 87 87 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="#1"		 binds [HCUEDKQNMYWSTRX]
BINDING 90 90 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="#1"		 binds [HCUEDKQNMYWSTRX]
BINDING 91 91 /ligand="Ca(2+)"
/ligand_id="ChEBI:CHEBI:29108"
/ligand_label="#1"		 binds [HCUEDKQNMYWSTRX]
end case
DISULFID 6 125 disulf C-x*-C
DISULFID 30 114 disulf C-x*-C
DISULFID 64 80 disulf C-x*-C
DISULFID 76 94 disulf C-x*-C

Additional information [?]

Size range 110-140 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

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UniProtKB rule member sequences [?]