ProRule PRU00700
General rule information
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Accession | PRU00700 |
Dates | 27-MAR-2008 (Created)
19-NOV-2022 (Last updated, Version 21) |
Data class | Domain; |
Predictors |
PROSITE; PS51368; UREASE_3 |
Name | Urease domain |
Function | Urease (EC 3.5.1.5) is a nickel-binding enzyme that catalyzes the hydrolysis of urea to carbon dioxide and ammonia. The urease domain forms an (alpha beta)(8) barrel structure that binds two nickel ions and with a histidine that is catalytically involved. |
Scope(s) |
Eukaryota Bacteria Archaea |
Example(s) | Q5KCQ6 (UREA_CRYNE); |
Propagated annotation
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Identifier, protein and gene names
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case <FT:2> and <FTGroup:1> and <FTGroup:2> and not <OC:Bacteria> and not <OC:Archaea> | |
Protein name | + AltName: Full=Urease; EC=3.5.1.5; AltName: Full=Urea amidohydrolase; |
end case |
Comments
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case <FT:2> and <FTGroup:1> and <FTGroup:2> | |
CATALYTIC ACTIVITY | Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5; |
end case | |
case <FTGroup:1> or <FTGroup:2> | |
COFACTOR | Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Note=Binds #(Ni(2+),ion) per subunit.; |
end case | |
case <OC:Bacteria> | |
SUBCELLULAR LOCATION | Cytoplasm. |
end case | |
case <OC:Eukaryota> and <AnyFeature:Nter~350,~PS51368> | |
SIMILARITY | In the C-terminal section; belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family. |
else | |
SIMILARITY | Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family. |
end case |
Keywords
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case <OC:Bacteria> | |
Cytoplasm | |
end case | |
case <FT:2> | |
Hydrolase | |
end case | |
case <FTGroup:1> or <FTGroup:2> | |
Metal-binding | |
Nickel | |
end case |
Gene Ontology
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case <FT:2> | |
GO:0016787; Molecular function:hydrolase activity | |
end case | |
case <FTGroup:1> or <FTGroup:2> | |
GO:0046872; Molecular function:metal ion binding | |
GO:0016151; Molecular function:nickel cation binding | |
end case | |
case <OCellular component:Bacteria> | |
GO:0005737; Cellular component:cytoplasm | |
end case |
Cross-references
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Features
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From: PS51368 | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | from | to | /note="Urease #" | |||||||||
ACT_SITE | 192 | 192 | /note="Proton donor" | H | ||||||||
BINDING | 6 | 6 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#1" |
H | 1 | |||||||
BINDING | 8 | 8 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#1" |
H | 1 | |||||||
BINDING | 89 | 89 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#1" /note="via carbamate group" |
K | 1 | |||||||
BINDING | 89 | 89 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#2" /note="via carbamate group" |
K | 1 | |||||||
BINDING | 118 | 118 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#2" |
H | 2 | |||||||
BINDING | 144 | 144 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#2" |
H | 2 | |||||||
BINDING | 232 | 232 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" /ligand_label="#1" |
D | 2 | |||||||
BINDING | 91 | 91 | /ligand="substrate" | H |
Additional information
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Size range | 420-470 amino acids |
Related rules |
None |
Fusion | None |
Repeats | 1 |
Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Archaea [4] Bacteria [261] Eukaryota [11] All [ 276 ]
- Retrieve set of proteins with 3D structure for this domain