We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU00772
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00772
General rule information
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| Accession | PRU00772 |
| Dates | 20-JUL-2010 (Created)
21-NOV-2019 (Last updated, Version 6) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51442; M_PRO |
Name | Coronavirus main protease (M-pro) domain |
| Function | It is a viral cysteine proteinase wich mediates most maturation cleavage events within the precursor polyprotein. |
| Scope(s) |
Viruses Coronavirinae |
| Example(s) | P0C6Y1; |
Propagated annotation
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Comments
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| case <FTGroup:1> | |
| FUNCTION | The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]- Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu- Gln-CMK. |
Keywords
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| Hydrolase | |
| Protease | |
| Thiol protease | |
| end case | |
Gene Ontology
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| GO:0019082; Biological process:viral protein processing |
| GO:0006508; Biological process:proteolysis |
| GO:0008233; Molecular function:peptidase activity |
| GO:0016787; Molecular function:hydrolase activity |
| GO:0008234; Molecular function:cysteine-type peptidase activity |
Features
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| From: PS51442 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="Peptidase C30 #" | |||||||||
| ACT_SITE | 41 | 41 | /note="For 3CL-PRO activity" | H | 1 | |||||||
| ACT_SITE | 144 | 144 | /note="For 3CL-PRO activity" | C | 1 | |||||||
Additional information
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| Size range | 292-317 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Viruses [59] All [ 59 ]
- Retrieve set of proteins with 3D structure for this domain