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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00773


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00773
General rule information [?]

Accession PRU00773
Dates 08-APR-2009 (Created)
17-FEB-2023 (Last updated, Version 11)
Data class Domain;
Predictors PROSITE; PS51443; PCS
Name Phytochelatin synthase (PCS) domain
Function Phytochelatins (PCs) are synthesized posttranslationally by the PC synthase (PCS) (EC 2.3.2.15), a gamma-glutamylcysteine (gamma-EC) transpeptidase. PC synthesis is proposed to have two distinct steps: (Step1) formation of gamma-EC concomitant with the cleavage of Gly from GSH; and (Step 2) transfer of the gamma-EC unit to an acceptor GSH molecule or an oligomeric PC peptide (PCn).
Scope(s) Eukaryota
Bacteria
Cyanobacteriota
Example(s) Q2TE74;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + AltName: Full=Glutathione gamma-glutamylcysteinyltransferase;
                 EC=2.3.2.15;
end case

Comments [?]

case <FTGroup:1>
CATALYTIC ACTIVITY Reaction=[Glu(-Cys)](n)-Gly + glutathione + H(+) = [Glu(-Cys)](n+1)-Gly + glycine; Xref=Rhea:RHEA:17917, Rhea:RHEA-COMP:12438, Rhea:RHEA- COMP:12439, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:57925, ChEBI:CHEBI:131728; EC=2.3.2.15;
end case
SIMILARITYBelongs to the phytochelatin synthase family.

Keywords [?]

Transferase
Acyltransferase
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0016756; Molecular function:glutathione gamma-glutamylcysteinyltransferase activity
GO:0046938; Biological process:phytochelatin biosynthetic process
GO:0016746; Molecular function:acyltransferase activity

Features [?]

From: PS51443
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase C83 #"
ACT_SITE 56 56 C 1
ACT_SITE 168 168 H 1
ACT_SITE 186 186 D 1

Additional information [?]

Size range 210-231 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]