We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU00780
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00780
General rule information
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| Accession | PRU00780 |
| Dates | 28-MAY-2009 (Created)
19-NOV-2022 (Last updated, Version 12) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51449; MTTASE_N PROSITE; PS01278; MTTASE_RADICAL |
Name | Methylthiotransferase domains |
| Function | Methylthiotransferases (MTTases) catalyze a C-H to C-S bond conversion in the methylthiolation of tRNA or ribosomal protein substrate. |
| Scope(s) |
Bacteria Eukaryota Archaea |
| Example(s) | Q54KV4; B4RNW8; |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Bacteria> or <OC:Archaea> | |
| Protein name | + RecName: EC=2.-.-.-; |
| end case | |
Comments
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| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| case <OC:Bacteria> | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
Keywords
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| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| S-adenosyl-L-methionine | |
| case <OC:Bacteria> or <OC:Archaea> | |
| Transferase | |
| end case | |
| case <OC:Bacteria> | |
| Cytoplasm | |
| end case | |
Gene Ontology
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| GO:0005506; Molecular function:iron ion binding | |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding | |
| case <OCellular component:Bacteria> or <OC:Archaea> | |
| GO:0016740; Molecular function:transferase activity | |
| end case | |
| case <OCellular component:Bacteria> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Features
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| From: PS51449 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="MTTase N-terminal #" | |||||||||
| BINDING | 10 | 10 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 46 | 46 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| BINDING | 77 | 77 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | ||||||||
| From: PS01278 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 4 | 4 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
|||||||||
| BINDING | 5 | 5 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
|||||||||
| BINDING | 8 | 8 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
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Additional information
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| Size range | 90-150 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Archaea [5] Bacteria [1031] Eukaryota [15] All [ 1051 ]
- Retrieve set of proteins with 3D structure for this domain