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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00902


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00902
General rule information [?]

Accession PRU00902
Dates 25-NOV-2011 (Created)
03-SEP-2024 (Last updated, Version 25)
Data class Domain;
Predictors PROSITE; PS51569; DOT1
Name Histone-lysine N-methyltransferase DOT1 (EC 2.1.1.43)
Function Histone-lysine N-methyltransferase (EC 2.1.1.43) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to L-lysine- [histone] to form N(6)-methyl-L-lysine-[histone] and S-adenosyl-L- homocysteine.
Scope(s) Eukaryota
Example(s) Q04089;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + RecName: EC=2.1.1.-;

Comments [?]

CATALYTIC ACTIVITY Reaction=L-lysyl-[histone] + S-adenosyl-L-methionine = N(6)-methyl-L- lysyl-[histone] + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:10024, Rhea:RHEA-COMP:9845, Rhea:RHEA-COMP:9846, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929;
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
end case

Keywords [?]

Methyltransferase
S-adenosyl-L-methionine
Transferase
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0032259; Biological process:methylation
GO:0042054; Molecular function:histone methyltransferase activity
GO:0016740; Molecular function:transferase activity
GO:0008168; Molecular function:methyltransferase activity
GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51569
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="DOT1 #"
case <FTGroup:1>
BINDING 120 123 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y-G-E-x
BINDING 143 152 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [FLV]-x-D-x-G-[ACS]-G-x-G-x
BINDING 206 207 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 x-F
end case
BINDING 170 170 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [DE] 1

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]