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ProRule PRU00924


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PURL: https://purl.expasy.org/prosite/rule/PRU00924
General rule information [?]

Accession PRU00924
Dates 25-NOV-2011 (Created)
16-JUN-2025 (Last updated, Version )
Data class Domain;
Predictors PROSITE; PS51591; RNA_CAP01_NS5_MT
Name mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57)
Function mRNA (guanine-N(7))-methyltransferase (EC 2.1.1.56) is an enzyme that transfers a methyl group from SAM to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and SAH. mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that transfers a methyl group from SAM to m(7)G(5')pppR-RNA to form m(7)G(5')pppRm-RNA and SAH.
Scope(s) Viruses
Flavivirus
Example(s) P12823; P06935; P03314;

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name + RecName: EC=2.1.1.56;
                 EC=2.1.1.57;

Comments [?]

CATALYTIC ACTIVITY Reaction=a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S- adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=2.1.1.56;
CATALYTIC ACTIVITY Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S- adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:67020, Rhea:RHEA- COMP:17167, Rhea:RHEA-COMP:17168, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167609; EC=2.1.1.57;
SIMILARITYIn the N-terminal section; belongs to the class I-like SAM- binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

Keywords [?]

Methyltransferase
mRNA capping
mRNA processing
RNA-binding
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0004482; Molecular function:mRNA 5'-cap (guanine-N7-)-methyltransferase activity
GO:0004483; Molecular function:methyltransferase cap1 activity
GO:0006370; Biological process:7-methylguanosine mRNA capping
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51591
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="mRNA cap 0-1 NS5-type MT #"
BINDING 56 56 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 S
BINDING 86 86 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 G
BINDING 87 87 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 W
BINDING 104 104 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 T
BINDING 105 105 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [ILK]
BINDING 131 131 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D
BINDING 132 132 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [IV]
BINDING 147 147 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 I
BINDING 220 220 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y
SITE 13 13 /note="mRNA cap binding" K
SITE 16 16 /note="mRNA cap binding; via carbonyl oxygen" L
SITE 17 17 /note="mRNA cap binding" N
SITE 19 19 /note="mRNA cap binding; via carbonyl oxygen" [CLM]
SITE 24 24 /note="mRNA cap binding" F
SITE 28 28 /note="mRNA cap binding" [KR]
SITE 61 61 /note="Essential for 2'-O-methyltransferase activity" K
SITE 146 146 /note="Essential for 2'-O-methyltransferase and N-7 methyltransferase activity" D
SITE 150 150 /note="mRNA cap binding" S
SITE 182 182 /note="Essential for 2'-O-methyltransferase activity" K
SITE 213 213 /note="mRNA cap binding" R
SITE 215 215 /note="mRNA cap binding" S
SITE 218 218 /note="Essential for 2'-O-methyltransferase activity" E

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]