We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.

ProRule PRU00924

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PURL: https://purl.expasy.org/prosite/rule/PRU00924

General rule information [?]

Accession	PRU00924
Dates	25-NOV-2011 (Created) 16-JUN-2025 (Last updated, Version 24)
Data class	Domain;
Predictors	PROSITE; PS51591; RNA_CAP01_NS5_MT
Name	mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57)
Function	mRNA (guanine-N(7))-methyltransferase (EC 2.1.1.56) is an enzyme that transfers a methyl group from SAM to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and SAH. mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that transfers a methyl group from SAM to m(7)G(5')pppR-RNA to form m(7)G(5')pppRm-RNA and SAH.
Scope(s)	Viruses Flavivirus
Example(s)	P12823; P06935; P03314;

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name

+ RecName: EC=2.1.1.56;
EC=2.1.1.57;

Comments [?]

CATALYTIC ACTIVITY	Reaction=a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S- adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=2.1.1.56;
CATALYTIC ACTIVITY	Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S- adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:67020, Rhea:RHEA- COMP:17167, Rhea:RHEA-COMP:17168, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167609; EC=2.1.1.57;
SIMILARITY	In the N-terminal section; belongs to the class I-like SAM- binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

Keywords [?]

S-adenosyl-L-methionine

Transferase

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding

GO:0004482; Molecular function:mRNA 5'-cap (guanine-N7-)-methyltransferase activity

GO:0004483; Molecular function:methyltransferase cap1 activity

GO:0006370; Biological process:7-methylguanosine mRNA capping

GO:0032259; Biological process:methylation

GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51591

Key

From

Description

Tag

Condition

FTGroup

DOMAIN

from

/note="mRNA cap 0-1 NS5-type MT #"

BINDING