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annotation rule: PRU00924

General rule information [?]

Accession PRU00924
Dates 25-NOV-2011 (Created)
19-JUL-2019 (Last updated, Version 15)
Data class Domain
Predictors PROSITE; PS51591; RNA_CAP01_NS5_MT
Name mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57)
Function mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56) is an enzyme that transfers a methyl group from SAM to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and SAH. mRNA (nucleoside-2'-O-)-methyltransferase (EC 2.1.1.57) is an enzyme that transfers a methyl group from SAM to m(7)G(5')pppR-RNA to form m(7)G(5')pppRm-RNA and SAH.

Propagated annotation [?]


Description [?]

+ RecName: EC=2.1.1.56; EC=2.1.1.57;

Comments [?]

Catalytic activity Reaction=a 5'-end (5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60856, Rhea:RHEA-COMP:15681, Rhea:RHEA-COMP:15683, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:143971, ChEBI:CHEBI:143975; EC=2.1.1.56;.
Reaction=a 5'-end (5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60852, Rhea:RHEA-COMP:15680, Rhea:RHEA-COMP:15682, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:143973, ChEBI:CHEBI:143974; EC=2.1.1.56;.
Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-guanosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-guanosine) in mRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60864, Rhea:RHEA-COMP:15683, Rhea:RHEA-COMP:15685, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:143975, ChEBI:CHEBI:143977; EC=2.1.1.57;.
Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-adenosine) in mRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60860, Rhea:RHEA-COMP:15682, Rhea:RHEA-COMP:15684, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:143974, ChEBI:CHEBI:143976; EC=2.1.1.57;.
Similarity In the N-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

Gene Ontology [?]

GO:0003723; Molecular function: RNA binding.
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity.
GO:0004483; Molecular function: mRNA (nucleoside-2'-O-)-methyltransferase activity.
GO:0006370; Biological process: 7-methylguanosine mRNA capping.
GO:0032259; Biological process: methylation.
GO:0046500; Biological process: S-adenosylmethionine metabolic process.

Keywords [?]


Features [?]

From: PS51591
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       mRNA cap 0-1 NS5-type MT #        
BINDING     56     56       S-adenosyl-L-methionine     S  
BINDING     86     86       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     87     87       S-adenosyl-L-methionine; via carbonyl oxygen     W  
BINDING     104     104       S-adenosyl-L-methionine     T  
BINDING     105     105       S-adenosyl-L-methionine; via carbonyl oxygen     [ILK]  
BINDING     131     131       S-adenosyl-L-methionine     D  
BINDING     132     132       S-adenosyl-L-methionine; via carbonyl oxygen     [IV]  
BINDING     147     147       S-adenosyl-L-methionine     I  
BINDING     220     220       S-adenosyl-L-methionine     Y  
SITE     13     13       mRNA cap binding     K  
SITE     16     16       mRNA cap binding; via carbonyl oxygen     L  
SITE     17     17       mRNA cap binding     N  
SITE     19     19       mRNA cap binding; via carbonyl oxygen     [CLM]  
SITE     24     24       mRNA cap binding     F  
SITE     28     28       mRNA cap binding     [KR]  
SITE     61     61       Essential for 2'-O-methyltransferase activity     K  
SITE     146     146       Essential for 2'-O-methyltransferase and N-7 methyltransferase activity     D  
SITE     150     150       mRNA cap binding     S  
SITE     182     182       Essential for 2'-O-methyltransferase activity     K  
SITE     213     213       mRNA cap binding     R  
SITE     215     215       mRNA cap binding     S  
SITE     218     218       Essential for 2'-O-methyltransferase activity     E  

Additional information [?]

Size range unlimited amino acids
Related rules None
Repeats 1
Topology Undefined
Examples P12823 (POLG_DEN2P); P06935 (POLG_WNV); P03314 (POLG_YEFV1): [Recover all]
Scope
Viruses; Flavivirus
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.



UniProtKB rule member sequences [?]