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annotation rule: PRU00924

General rule information [?]

Accession PRU00924
Dates 25-NOV-2011 (Created)
27-OCT-2018 (Last updated, Version 12)
Data class Domain
Predictors PROSITE; PS51591; RNA_CAP01_NS5_MT
Name mRNA cap 0 and cap 1 methyltransferase (EC and EC
Function mRNA (guanine-N(7)-)-methyltransferase (EC is an enzyme that transfers a methyl group from SAM to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and SAH. mRNA (nucleoside-2'-O-)-methyltransferase (EC is an enzyme that transfers a methyl group from SAM to m(7)G(5')pppR-RNA to form m(7)G(5')pppRm-RNA and SAH.

Propagated annotation [?]

Description [?]

+ RecName: EC=; EC=;

Comments [?]

Catalytic activity Reaction=a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)- [mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'- triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S- adenosyl-L-homocysteine; Xref=Rhea:RHEA:11220, Rhea:RHEA- COMP:12925, Rhea:RHEA-COMP:13407, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:133968, ChEBI:CHEBI:136896; EC=;.
Reaction=a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine- ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)- methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine- ribonucleotide)-[mRNA] + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:19617, Rhea:RHEA-COMP:12925, Rhea:RHEA- COMP:12926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:133968, ChEBI:CHEBI:133969; EC=;.
Similarity In the N-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

Gene Ontology [?]

GO:0003723; Molecular function: RNA binding.
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity.
GO:0004483; Molecular function: mRNA (nucleoside-2'-O-)-methyltransferase activity.
GO:0006370; Biological process: 7-methylguanosine mRNA capping.
GO:0032259; Biological process: methylation.
GO:0046500; Biological process: S-adenosylmethionine metabolic process.

Keywords [?]

Features [?]

From: PS51591
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       mRNA cap 0-1 NS5-type MT #        
BINDING     56     56       S-adenosyl-L-methionine     S  
BINDING     86     86       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     87     87       S-adenosyl-L-methionine; via carbonyl oxygen     W  
BINDING     104     104       S-adenosyl-L-methionine     T  
BINDING     105     105       S-adenosyl-L-methionine; via carbonyl oxygen     [ILK]  
BINDING     131     131       S-adenosyl-L-methionine     D  
BINDING     132     132       S-adenosyl-L-methionine; via carbonyl oxygen     [IV]  
BINDING     147     147       S-adenosyl-L-methionine     I  
BINDING     220     220       S-adenosyl-L-methionine     Y  
SITE     13     13       mRNA cap binding     K  
SITE     16     16       mRNA cap binding; via carbonyl oxygen     L  
SITE     17     17       mRNA cap binding     N  
SITE     19     19       mRNA cap binding; via carbonyl oxygen     [CLM]  
SITE     24     24       mRNA cap binding     F  
SITE     28     28       mRNA cap binding     [KR]  
SITE     61     61       Essential for 2'-O-methyltransferase activity     K  
SITE     146     146       Essential for 2'-O-methyltransferase and N-7 methyltransferase activity     D  
SITE     150     150       mRNA cap binding     S  
SITE     182     182       Essential for 2'-O-methyltransferase activity     K  
SITE     213     213       mRNA cap binding     R  
SITE     215     215       mRNA cap binding     S  
SITE     218     218       Essential for 2'-O-methyltransferase activity     E  

Additional information [?]

Size range unlimited amino acids
Related rules None
Repeats 1
Topology Undefined
Examples P12823 (POLG_DEN2P); P06935 (POLG_WNV); P03314 (POLG_YEFV1): [Recover all]
Viruses; Flavivirus
Comments None


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UniProtKB rule member sequences [?]