ProRule PRU00924

General rule information [?]

Accession PRU00924
Dates 25-NOV-2011 (Created)
11-JAN-2024 (Last updated, Version 21)
Data class Domain;
Predictors PROSITE; PS51591; RNA_CAP01_NS5_MT
Name mRNA cap 0 and cap 1 methyltransferase (EC and EC
Function mRNA (guanine-N(7))-methyltransferase (EC is an enzyme that transfers a methyl group from SAM to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and SAH. mRNA (nucleoside-2'-O-)-methyltransferase (EC is an enzyme that transfers a methyl group from SAM to m(7)G(5')pppR-RNA to form m(7)G(5')pppRm-RNA and SAH.
Scope(s) Viruses
Example(s) P12823 (POLG_DEN2P); P06935 (POLG_WNV); P03314 (POLG_YEFV1);

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name + RecName: EC=;

Comments [?]

CATALYTIC ACTIVITY Reaction=a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S- adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=;
CATALYTIC ACTIVITY Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + H(+) + S- adenosyl-L-homocysteine; Xref=Rhea:RHEA:67020, Rhea:RHEA-COMP:17167, Rhea:RHEA-COMP:17168, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167609; EC=;
SIMILARITYIn the N-terminal section; belongs to the class I-like SAM- binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.

Keywords [?]

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0004482; Molecular function:mRNA 5'-cap (guanine-N7-)-methyltransferase activity
GO:0004483; Molecular function:mRNA (nucleoside-2'-O-)-methyltransferase activity
GO:0006370; Biological process:7-methylguanosine mRNA capping
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51591
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="mRNA cap 0-1 NS5-type MT #"
BINDING 56 56 /ligand="S-adenosyl-L-methionine"
BINDING 86 86 /ligand="S-adenosyl-L-methionine"
BINDING 87 87 /ligand="S-adenosyl-L-methionine"
BINDING 104 104 /ligand="S-adenosyl-L-methionine"
BINDING 105 105 /ligand="S-adenosyl-L-methionine"
BINDING 131 131 /ligand="S-adenosyl-L-methionine"
BINDING 132 132 /ligand="S-adenosyl-L-methionine"
BINDING 147 147 /ligand="S-adenosyl-L-methionine"
BINDING 220 220 /ligand="S-adenosyl-L-methionine"
SITE 13 13 /note="mRNA cap binding" K
SITE 16 16 /note="mRNA cap binding; via carbonyl oxygen" L
SITE 17 17 /note="mRNA cap binding" N
SITE 19 19 /note="mRNA cap binding; via carbonyl oxygen" [CLM]
SITE 24 24 /note="mRNA cap binding" F
SITE 28 28 /note="mRNA cap binding" [KR]
SITE 61 61 /note="Essential for 2'-O-methyltransferase activity" K
SITE 146 146 /note="Essential for 2'-O-methyltransferase and N-7 methyltransferase activity" D
SITE 150 150 /note="mRNA cap binding" S
SITE 182 182 /note="Essential for 2'-O-methyltransferase activity" K
SITE 213 213 /note="mRNA cap binding" R
SITE 215 215 /note="mRNA cap binding" S
SITE 218 218 /note="Essential for 2'-O-methyltransferase activity" E

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


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UniProtKB rule member sequences [?]