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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00932


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00932
General rule information [?]

Accession PRU00932
Dates 14-DEC-2011 (Created)
03-SEP-2024 (Last updated, Version 15)
Data class Domain;
Predictors PROSITE; PS51600; SAM_GNMT
Name Glycine and glycine/sarcosine N-methyltransferase
Function Glycine N-methyltransferase (EC 2.1.1.20) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to glycine to form sarcosine and S-adenosyl-L-homocysteine. Glycine/sarcosine N-methyltransferase (EC 2.1.1.156) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to glycine to form sarcosine and S-adenosyl-L-homocysteine AND transfers a methyl group from S-adenosyl-L-methionine (SAM) to sarcosine to form N,N-dimethylglycine and S-adenosyl-L-homocysteine.
Scope(s) Eukaryota
Eutheria
Bacteria
Example(s) P13255;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and (<OC:Bacteria> or <OC:Archaea>)
Protein name + RecName: EC=2.1.1.156;
else case <FTGroup:1>
Protein name + RecName: EC=2.1.1.20;
end case

Comments [?]

case <FTGroup:1> and (<OC:Bacteria> or <OC:Archaea>)
CATALYTIC ACTIVITY Reaction=glycine + 2 S-adenosyl-L-methionine = N,N-dimethylglycine + 2 S-adenosyl-L-homocysteine + 2 H(+); Xref=Rhea:RHEA:32463, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:57856, ChEBI:CHEBI:58251, ChEBI:CHEBI:59789; EC=2.1.1.156;
else case <FTGroup:1>
CATALYTIC ACTIVITY Reaction=glycine + S-adenosyl-L-methionine = sarcosine + S-adenosyl-L- homocysteine + H(+); Xref=Rhea:RHEA:19937, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:57433, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.20;
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Keywords [?]

case <FTGroup:1>
Methyltransferase
S-adenosyl-L-methionine
Transferase
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0017174; Molecular function:glycine N-methyltransferase activity
end case
case <FTGroup:1> and (<OCellular component:Bacteria> or <OC:Archaea>)
GO:0052730; Molecular function:sarcosine N-methyltransferase activity
end case
case <FTGroup:1>
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process
end case

Features [?]

From: PS51600
Key From To Description Tag Condition FTGroup
case <FTGroup:1>
BINDING 122 123 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [DN]-W
end case
BINDING 27 27 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y 1
case <FTGroup:1>
BINDING 36 36 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [WY]
BINDING 39 39 /ligand="substrate" Y
BINDING 46 46 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 R
BINDING 70 70 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [AS]
BINDING 91 91 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D
BINDING 143 143 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [FMLQ]
BINDING 145 145 /ligand="substrate" [NS]
end case
BINDING 179 179 /ligand="substrate" R 1
case <FTGroup:1>
BINDING 223 223 /ligand="substrate"
end case

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]