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ProRule PRU00932


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PURL: https://purl.expasy.org/prosite/rule/PRU00932
General rule information [?]

Accession PRU00932
Dates 14-DEC-2011 (Created)
03-SEP-2024 (Last updated, Version 15)
Data class Domain;
Predictors PROSITE; PS51600; SAM_GNMT
Name Glycine and glycine/sarcosine N-methyltransferase
Function Glycine N-methyltransferase (EC 2.1.1.20) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to glycine to form sarcosine and S-adenosyl-L-homocysteine. Glycine/sarcosine N-methyltransferase (EC 2.1.1.156) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to glycine to form sarcosine and S-adenosyl-L-homocysteine AND transfers a methyl group from S-adenosyl-L-methionine (SAM) to sarcosine to form N,N-dimethylglycine and S-adenosyl-L-homocysteine.
Scope(s) Eukaryota
Eutheria
Bacteria
Example(s) P13255 (GNMT_RAT);

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and (<OC:Bacteria> or <OC:Archaea>)
Protein name + RecName: EC=2.1.1.156;
else case <FTGroup:1>
Protein name + RecName: EC=2.1.1.20;
end case

Comments [?]

case <FTGroup:1> and (<OC:Bacteria> or <OC:Archaea>)
CATALYTIC ACTIVITY Reaction=glycine + 2 S-adenosyl-L-methionine = N,N-dimethylglycine + 2 S-adenosyl-L-homocysteine + 2 H(+); Xref=Rhea:RHEA:32463, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:57856, ChEBI:CHEBI:58251, ChEBI:CHEBI:59789; EC=2.1.1.156;
else case <FTGroup:1>
CATALYTIC ACTIVITY Reaction=glycine + S-adenosyl-L-methionine = sarcosine + S-adenosyl-L- homocysteine + H(+); Xref=Rhea:RHEA:19937, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305, ChEBI:CHEBI:57433, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.20;
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Keywords [?]

case <FTGroup:1>
Methyltransferase
S-adenosyl-L-methionine
Transferase
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0017174; Molecular function:glycine N-methyltransferase activity
end case
case <FTGroup:1> and (<OCellular component:Bacteria> or <OC:Archaea>)
GO:0052730; Molecular function:sarcosine N-methyltransferase activity
end case
case <FTGroup:1>
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process
end case

Features [?]

From: PS51600
Key From To Description Tag Condition FTGroup
case <FTGroup:1>
BINDING 122 123 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [DN]-W
end case
BINDING 27 27 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y 1
case <FTGroup:1>
BINDING 36 36 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [WY]
BINDING 39 39 /ligand="substrate" Y
BINDING 46 46 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 R
BINDING 70 70 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [AS]
BINDING 91 91 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D
BINDING 143 143 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 [FMLQ]
BINDING 145 145 /ligand="substrate" [NS]
end case
BINDING 179 179 /ligand="substrate" R 1
case <FTGroup:1>
BINDING 223 223 /ligand="substrate"
end case

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

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UniProtKB rule member sequences [?]