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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00952


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00952
General rule information [?]

Accession PRU00952
Dates 14-DEC-2011 (Created)
03-SEP-2024 (Last updated, Version 16)
Data class Domain;
Predictors PROSITE; PS51620; SAM_TRM61
Name tRNA (adenine(57)-N(1)/adenine(58)-N(1) or adenine(58)-N(1))-
Function tRNA (adenine(58)-N(1))-methyltransferase (EC 2.1.1.220) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to adenine(58) in tRNA to form N(1)-methyladenine(58) in tRNA and S-adenosyl-L- homocysteine. tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase (EC=2.1.1.219) is an enzyme that transfers a methyl group from the S-adenosyl-L-methionine (SAM) to adenine(57)/adenine(58) in tRNA to form N(1)-methyladenine57/N(1)- methyladenine58 in tRNA and S-adenosyl-L- homocysteine.
Scope(s) Eukaryota
Example(s) Q755M8;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Archaea>
Protein name + RecName: EC=2.1.1.219;
else
Protein name + RecName: EC=2.1.1.220;
end case

Comments [?]

case <OC:Archaea>
CATALYTIC ACTIVITY Reaction=adenosine(57)/adenosine(58) in tRNA + 2 S-adenosyl-L- methionine = N(1)-methyladenosine(57)/N(1)-methyladenosine(58) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+); Xref=Rhea:RHEA:41740, Rhea:RHEA-COMP:9580, Rhea:RHEA-COMP:9582, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74491; EC=2.1.1.219;
else
CATALYTIC ACTIVITY Reaction=adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)- methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:43152, Rhea:RHEA-COMP:10365, Rhea:RHEA-COMP:10366, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74491; EC=2.1.1.220;
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.

Keywords [?]

Methyltransferase
S-adenosyl-L-methionine
Transferase
tRNA processing

Gene Ontology [?]

case <OCellular component:Archaea>
GO:0043827; Molecular function:tRNA (adenine(57)-N1)/(adenine(58)-N1)-methyltransferase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0160107; Molecular function:tRNA (adenine(58)-N1)-methyltransferase activity
end case
GO:0032259; Biological process:methylation

Features [?]

From: PS51620
Key From To Description Tag Condition FTGroup
BINDING 102 102 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 S
BINDING 123 123 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 E
BINDING 151 151 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D
BINDING 170 170 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]