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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00955


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00955
General rule information [?]

Accession PRU00955
Dates 14-DEC-2011 (Created)
03-SEP-2024 (Last updated, Version 12)
Data class Domain;
Predictors
case c? <OC:Fungi> and <Length>=1149> and <Length<=1723>
PROSITE; PS51623; SAM_MT_TRMH_1
end case
Name tRNA guanosine-2'-O-methyltransferase
Function tRNA guanosine-2'-O-methyltransferase (EC 2.1.1.34) is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to tRNA to form tRNA containing 2'-O-methylguanosine and S-adenosyl-L-homocysteine.
Scope(s) Eukaryota
Saccharomyces
Example(s) Q07527;

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name + RecName: EC=2.1.1.34;

Comments [?]

CATALYTIC ACTIVITY Reaction=guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O- methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:20077, Rhea:RHEA-COMP:10190, Rhea:RHEA-COMP:10192, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74445; EC=2.1.1.34;
SIMILARITYBelongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.

Keywords [?]

Methyltransferase
RNA-binding
S-adenosyl-L-methionine
Transferase
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0141100; Molecular function:tRNA (guanine(18)-2'-O)-methyltransferase activity
GO:0008033; Biological process:tRNA processing
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51623
Key From To Description Tag Condition FTGroup
BINDING 1389 1389 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 G
BINDING 1409 1409 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 I

Additional information [?]

Size range unlimited amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]