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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00958


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00958
General rule information [?]

Accession PRU00958
Dates 14-DEC-2011 (Created)
03-SEP-2024 (Last updated, Version 17)
Data class Domain;
Predictors PROSITE; PS51626; SAM_MT_TRM1
Name tRNA (guanine(26)-N(2))-dimethyltransferase (EC 2.1.1.216)
Function tRNA (guanine(26)-N(2))-dimethyltransferase (EC 2.1.1.216) is an enzyme that transfers a methyl group from 2 S-adenosyl-L-methionine (SAM) to guanine(26) in tRNA to form N(2)-dimethylguanine(26) in tRNA and 2 S- adenosyl-L-homocysteine.
Scope(s) Archaea
Eukaryota
Bacteria
Aquifex
Example(s) Q9LFU5;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Eukaryota> and <Length>=425> and <Length<=670>
Protein name + RecName: EC=2.1.1.216;

Comments [?]

CATALYTIC ACTIVITY Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)- dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+); Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.

Keywords [?]

Methyltransferase
RNA-binding
S-adenosyl-L-methionine
Transferase
tRNA processing
tRNA-binding

Gene Ontology [?]

case <OCellular component:Eukaryota> and <Length>=425> and <Length<=670>
GO:0160104; Molecular function:tRNA (guanine(26)-N2)-dimethyltransferase activity
end case
GO:0000049; Molecular function:tRNA binding
GO:0008033; Biological process:tRNA processing
GO:0032259; Biological process:methylation

Features [?]

From: PS51626
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Trm1 methyltransferase #"

Additional information [?]

Size range 58-472 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]