We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU00995
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00995
General rule information
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| Accession | PRU00995 |
| Dates | 14-JAN-2013 (Created)
19-NOV-2022 (Last updated, Version 25) |
| Data class | Domain; |
| Predictors |
PROSITE; PS50880; TOPRIM |
Name | Toprim domain |
| Function | The Toprim domain is a catalytic domain involved in DNA strand breakage and rejoining. |
| Scope(s) |
Eukaryota Bacteria Archaea Viruses |
| Example(s) | P06786; |
Propagated annotation
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Identifier, protein and gene names
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| case <FTGroup:1> and <FTGroup:2> | |
| Protein name | + RecName: EC=5.6.2.2; |
Comments
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| CATALYTIC ACTIVITY | Reaction=ATP-dependent breakage, passage and rejoining of double- stranded DNA.; EC=5.6.2.2; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).; |
| else case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
Keywords
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| DNA-binding | |
| end case | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| case <FTGroup:2> | |
| GO:0003677; Molecular function:DNA binding | |
Features
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| From: PS50880 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="Toprim #" | |||||||||
| BINDING | 7 | 7 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#1" /ligand_note="catalytic" |
E | 1 | |||||||
| BINDING | 65 | 65 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#1" /ligand_note="catalytic" |
D | 1 | |||||||
| SITE | 38 | 38 | /note="Interaction with DNA" | K | 2 | |||||||
| SITE | 41 | 41 | /note="Interaction with DNA" | N | 2 | |||||||
Additional information
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| Size range | 105-128 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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