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annotation rule: PRU00995

General rule information [?]

Accession PRU00995
Dates 14-JAN-2013 (Created)
21-NOV-2019 (Last updated, Version 20)
Data class Domain
Predictors PROSITE; PS50880; TOPRIM
Name Toprim domain
Function The Toprim domain is a catalytic domain involved in DNA strand breakage and rejoining.

Propagated annotation [?]


Description [?]

case <FTGroup:1> and <FTGroup:2>
+ RecName: EC 5.6.2.2;

Comments [?]

Catalytic activity Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2;
Cofactor Mg(2+)
Mn(2+)
Ca(2+)
Note: Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).
else case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds two Mg(2+) per subunit.
end case


Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case

case <FTGroup:2>
GO:0003677; Molecular function: DNA binding.

Keywords [?]

DNA-binding
end case

case <FTGroup:1>
Magnesium
Metal-binding
end case


Features [?]

From: PS50880
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       Toprim #        
METAL     7     7       Magnesium 1; catalytic     E   1
METAL     65     65       Magnesium 1; catalytic     D   1
METAL     65     65       Magnesium 2     D   1
METAL     67     67       Magnesium 2     D   1
SITE     38     38       Interaction with DNA     K   2
SITE     41     41       Interaction with DNA     N   2

Additional information [?]

Size range 105-128 amino acids
Related rules None
Repeats 1
Topology Undefined
Example P06786 (TOP2_YEAST)
Scope
Eukaryota
Bacteria
Archaea
Viruses
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


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UniProtKB rule member sequences [?]