We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU01029
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01029
General rule information
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| Accession | PRU01029 |
| Dates | 11-NOV-2013 (Created)
30-SEP-2022 (Last updated, Version 10) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51692; PESTIVIRUS_NS2_PRO |
Name | Pestivirus nonstructural protein 2 (NS2) protease domain |
| Function | The C-terminal-most domain of NS2 is a cysteine autoprotease responsible for NS2-3 cleavage. The catalytic triad is formed by His, Glu, and Cys. The pestivirus NS2 protease domain contains five Cys and one His organized in a linear fashion, suggesting the presence of a mononuclear ZnB site. The pestivirus NS2 protease domain forms peptidase family C74. |
| Scope(s) |
Viruses Pestivirus |
| Example(s) | P19711; |
Propagated annotation
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Identifier, protein and gene names
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| case <FTGroup:1> | |
| Protein name | + Contains: RecName: Full=Cysteine protease NS2; EC=3.4.22.-; |
Comments
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| FUNCTION | NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. |
| SUBCELLULAR LOCATION | Cysteine protease NS2: Host membrane; Multi-pass membrane protein. |
| end case | |
Keywords
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| Host membrane | |
| case <FTGroup:1> | |
| Hydrolase | |
| end case | |
| Membrane | |
| case <FTGroup:1> | |
| Protease | |
| Thiol protease | |
| end case | |
| Transmembrane | |
| Transmembrane helix | |
Gene Ontology
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| GO:0016020; Cellular component:membrane | |
| case <FTGroup:1> | |
| GO:0019082; Biological process:viral protein processing | |
| GO:0006508; Biological process:proteolysis | |
| end case | |
| GO:0033644; Cellular component:host cell membrane | |
| case <FTGroup:1> | |
| GO:0004197; Molecular function:cysteine-type endopeptidase activity | |
| end case | |
Features
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| From: PS51692 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| TRANSMEM | 128 | 148 | /note="Helical" | |||||||||
| DOMAIN | from | to | /note="Peptidase C74 #" | |||||||||
| ACT_SITE | 7 | 7 | /note="For cysteine protease NS2 activity" | H | 1 | |||||||
| ACT_SITE | 21 | 21 | /note="For cysteine protease NS2 activity" | E | 1 | |||||||
| ACT_SITE | 72 | 72 | /note="For cysteine protease NS2 activity" | C | 1 | |||||||
| case <FTGroup:1> | ||||||||||||
| CHAIN | ? | 149 | /note="Cysteine protease NS2" | |||||||||
| SITE | 149 | 149+1 | /note="Cleavage; partial; cysteine protease NS2" | |||||||||
| end case | ||||||||||||
Additional information
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| Size range | 139-249 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Viruses [5] All [ 5 ]
- Retrieve set of proteins with 3D structure for this domain