PRU01029

General rule information [?]

Accession	PRU01029
Dates	11-NOV-2013 (Created) 30-SEP-2022 (Last updated, Version 9)

Data class

Domain

Predictors

PROSITE; PS51692; PESTIVIRUS_NS2_PRO

Name	Pestivirus nonstructural protein 2 (NS2) protease domain

Function

The C-terminal-most domain of NS2 is a cysteine autoprotease responsible for NS2-3 cleavage. The catalytic triad is formed by His, Glu, and Cys. The pestivirus NS2 protease domain contains five Cys and one His organized in a linear fashion, suggesting the presence of a mononuclear ZnB site. The pestivirus NS2 protease domain forms peptidase family C74.

Propagated annotation [?]

Description [?]

case <FTGroup:1>

+ Contains: RecName: Full=Cysteine protease NS2; EC 3.4.22.-;

Comments [?]

Function	NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
Subcellular location	Cysteine protease NS2: Host membrane; Multi-pass membrane protein.

end case

Gene Ontology [?]

GO:0016020; Cellular component: membrane.

case <FTGroup:1>

GO:0019082; Biological process: viral protein processing.
GO:0006508; Biological process: proteolysis.

end case

GO:0033644; Cellular component: host cell membrane.

case <FTGroup:1>

GO:0004197; Molecular function: cysteine-type endopeptidase activity.

end case

Keywords [?]

Host membrane

case <FTGroup:1>

Hydrolase

end case

Membrane

case <FTGroup:1>

Protease
Thiol protease

end case

Transmembrane
Transmembrane helix

Features [?]

From: PS51692

Key From To Description Tag Condition FTGroup

TRANSMEM 128 148 Helical

DOMAIN from to Peptidase C74 #

ACT_SITE 7 7 For cysteine protease NS2 activity H 1

ACT_SITE 21 21 For cysteine protease NS2 activity E 1

ACT_SITE 72 72 For cysteine protease NS2 activity C 1

case <FTGroup:1>

CHAIN ? 149 Cysteine protease NS2

SITE 149 149+1 Cleavage; partial; cysteine protease NS2

end case

Additional information [?]

Size range	139-249 amino acids
Related rules	None
Repeats	1
Topology	Undefined
Example	P19711 (POLG_BVDVN)
Scope	Viruses; Pestivirus
Comments	None

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UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Viruses

[5]