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annotation rule: PRU01029

General rule information [?]

Accession PRU01029
Dates 11-NOV-2013 (Created)
22-NOV-2019 (Last updated, Version 8)
Data class Domain
Predictors PROSITE; PS51692; PESTIVIRUS_NS2_PRO
Name Pestivirus nonstructural protein 2 (NS2) protease domain
Function The C-terminal-most domain of NS2 is a cysteine autoprotease responsible for NS2-3 cleavage. The catalytic triad is formed by His, Glu, and Cys. The pestivirus NS2 protease domain contains five Cys and one His organized in a linear fashion, suggesting the presence of a mononuclear ZnB site. The pestivirus NS2 protease domain forms peptidase family C74.

Propagated annotation [?]


Description [?]

case <FTGroup:1>
+ Contains: RecName: Full=Cysteine protease NS2; EC 3.4.22.-;

Comments [?]

Function NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
Subcellular location Cysteine protease NS2: Host membrane; Multi-pass membrane protein.
end case


Gene Ontology [?]

GO:0016021; Cellular component: integral component of membrane.
case <FTGroup:1>
GO:0019082; Biological process: viral protein processing.
GO:0006508; Biological process: proteolysis.
end case

GO:0033644; Cellular component: host cell membrane.
case <FTGroup:1>
GO:0004197; Molecular function: cysteine-type endopeptidase activity.
end case


Keywords [?]

case <FTGroup:1>
end case

case <FTGroup:1>
end case


Features [?]

From: PS51692
Key     From     To       Description   Tag   Condition   FTGroup
TRANSMEM     128     148       Helical        
DOMAIN     from     to       Peptidase C74 #        
ACT_SITE     7     7       For cysteine protease NS2 activity     H   1
ACT_SITE     21     21       For cysteine protease NS2 activity     E   1
ACT_SITE     72     72       For cysteine protease NS2 activity     C   1
case <FTGroup:1>
CHAIN     ?     149       Cysteine protease NS2        
SITE     149     149+1       Cleavage; partial; cysteine protease NS2        
end case


Additional information [?]

Size range 139-249 amino acids
Related rules None
Repeats 1
Topology Undefined
Example P19711 (POLG_BVDVN)
Scope
Viruses; Pestivirus
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.



UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets
Viruses [5]