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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01197


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PURL: https://purl.expasy.org/prosite/rule/PRU01197
General rule information [?]

Accession PRU01197
Dates 02-JAN-2018 (Created)
05-NOV-2024 (Last updated, Version 7)
Data class Domain;
Predictors PROSITE; PS51850; KARI_N
Name KARI N-terminal domain
Function Ketol-acid reductoisomerase (KARI; EC 1.1.1.86), also known as acetohydroxy acid isomeroreductase (AHIR or AHAIR), catalyzes the conversion of acetohydroxy acids into dihydroxy valerates in the second step of the biosynthetic pathway for the essential branched-chain amino acids valine, leucine, and isoleucine. KARIs are composed of two types of domains, an N- terminal Rossmann fold domain and one or two C-terminal knotted domains.
Scope(s) Bacteria
Archaea
Eukaryota
Example(s) A7GMU0;

Propagated annotation [?]

Comments [?]

SIMILARITYBelongs to the ketol-acid reductoisomerase family.

Keywords [?]

NADP
end case
Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Oxidoreductase

Gene Ontology [?]

GO:0004455; Molecular function:ketol-acid reductoisomerase activity
GO:0009097; Biological process:isoleucine biosynthetic process
GO:0009099; Biological process:L-valine biosynthetic process
case <FTGroup:1>
GO:0050661; Molecular function:NADP binding

Features [?]

From: PS51850
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="KARI N-terminal Rossmann #"
BINDING 27 30 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 1
ACT_SITE 110 110 H
BINDING 50 50 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 R 1
BINDING 55 55 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 S 1
BINDING 136 136 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 G 1

Additional information [?]

Size range 135-205 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]