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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01233


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01233
General rule information [?]

Accession PRU01233
Dates 07-FEB-2019 (Created)
19-NOV-2022 (Last updated, Version 5)
Data class Domain;
Predictors PROSITE; PS51885; NEPRILYSIN
Name Neprilysin-like (M13) protease domain
Function The neprilysin (M13) family of endopeptidases are zinc- metalloenzymes, the majority of which are type II integral membrane proteins with their active sites facing the extracellular environment.
Scope(s) Eukaryota
Metazoa
Bacteria
Lactobacillales
Viruses
Mimivirus
Example(s) P0C2B4;

Propagated annotation [?]

Comments [?]

SIMILARITYBelongs to the peptidase M13 family.

Keywords [?]

Hydrolase
Metalloprotease
Protease
end case
case <FTGroup:2>
Metal-binding
Zinc
end case
case <FTTag:disulf>
Disulfide bond
end case

Gene Ontology [?]

GO:0004222; Molecular function:metalloendopeptidase activity

Cross-references [?]

PROSITE PS00142; ZINC_PROTEASE; 1;

Features [?]

From: PS51885
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase M13 #"
ACT_SITE 510 510 E 1
ACT_SITE 575 575 /note="Proton donor" D 1
BINDING 509 509 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H 2
BINDING 513 513 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H 2
BINDING 571 571 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 E 2
DISULFID 2 7 disulf C-x*-C
DISULFID 25 659 disulf C-x*-C
DISULFID 33 619 disulf C-x*-C
DISULFID 89 338 disulf C-x*-C
DISULFID 175 183 disulf C-x*-C
DISULFID 546 671 disulf C-x*-C

Additional information [?]

Size range 600-800 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]