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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01237


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01237
General rule information [?]

Accession PRU01237
Dates 05-APR-2019 (Created)
19-NOV-2022 (Last updated, Version 5)
Data class Domain;
Predictors PROSITE; PS51889; RUBV_NS_PRO
Name Rubella virus (RUBV) nonstructural (NS) protease domain
Function This RUBV NS protease domain is a papain-like protease with catalytic dyad of Cys and His.
Scope(s) Viruses
Rubivirus
Example(s) Q86500;

Propagated annotation [?]

Comments [?]

case <FTGroup:2>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Zn(2+) is necessary for the protease activity. The protease can also function efficiently with Cd(2+) and Co(2+)
end case
PTMNon-structural polyprotein p200: Specific enzymatic cleavage by its own cysteine protease yield mature proteins p150 and p90.

Keywords [?]

Hydrolase
Protease
Thiol protease
end case
case <FTGroup:2>
Metal-binding
Zinc
end case

Gene Ontology [?]

case <FTGroup:2>
GO:0046872; Molecular function:metal ion binding
end case
case <FTGroup:1>
GO:0004197; Molecular function:cysteine-type endopeptidase activity

Features [?]

From: PS51889
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase C27 #"
REGION 153 184 /note="Interaction with host CALM1"
REGION 194 229 /note="EF-hand-like"
ACT_SITE 153 153 /note="For cysteine protease activity" C 1
ACT_SITE 274 274 /note="For cysteine protease activity" H 1
BINDING 176 176 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 2
BINDING 179 179 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 2
BINDING 228 228 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 2
BINDING 274 274 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 2
case <FTGroup:1>
SITE to to+1 /note="Cleavage; autocatalytic" G-G
end case

Additional information [?]

Size range 295-310 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]