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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01319


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01319
General rule information [?]

Accession PRU01319
Dates 05-MAY-2021 (Created)
19-NOV-2022 (Last updated, Version 5)
Data class Domain;
Predictors PROSITE; PS51975; RNASE_H_2
Name RNase H type-2 domain
Function Ribonuclease H (RNase H) (EC 3.1.26.4) recognizes and cleaves the RNA strand of RNA-DNA heteroduplexes.
Scope(s) Bacteria
Archaea
Eukaryota
Example(s) B8DHN2;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + RecName: EC=3.1.26.4;

Comments [?]

CATALYTIC ACTIVITY Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
COFACTOR Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.
end case

Keywords [?]

Endonuclease
Hydrolase
Metal-binding
Nuclease
end case

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
case <FTGroup:1>
GO:0004523; Molecular function:RNA-DNA hybrid ribonuclease activity
GO:0006401; Biological process:RNA catabolic process

Features [?]

From: PS51975
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="RNase H type-2"
BINDING 7 7 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D 1
BINDING 8 8 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 E 1
BINDING 105 105 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D 1

Additional information [?]

Size range 170-235 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]