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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01362


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01362
General rule information [?]

Accession PRU01362
Dates 21-FEB-2023 (Created)
03-SEP-2024 (Last updated, Version 3)
Data class Domain;
Predictors PROSITE; PS52018; DART
Name DNA ADP-ribosyl transferase (DarT) domain
Function The bacterial DNA ADP-ribosyl transferase (DarT) protein is a toxin that has been shown to transfer ADP-ribose from NAD(+) onto thymidine bases present in single-stranded DNA (ssDNA) specifically at the four-base motif TNTC, and to have no activity on RNA or protein targets.
Scope(s) Bacteria
Example(s) A0A0B0SG80;

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
CATALYTIC ACTIVITY Reaction=a thymidine in DNA + NAD(+) = an N-(ADP-alpha-D-ribosyl)- thymidine in DNA + nicotinamide + H(+); Xref=Rhea:RHEA:71651, Rhea:RHEA-COMP:13556, Rhea:RHEA-COMP:18051, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:137386, ChEBI:CHEBI:191199; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71652;
end case
SIMILARITYBelongs to the DarT ADP-ribosyltransferase family.

Keywords [?]

Glycosyltransferase
Nucleotidyltransferase
Transferase
end case
DNA-binding
Toxin-antitoxin system

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
case <FTGroup:1>
GO:0016779; Molecular function:nucleotidyltransferase activity
GO:0016757; Molecular function:glycosyltransferase activity

Features [?]

From: PS52018
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="DarT"
BINDING 5 7 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 1
case <FTGroup:1>
BINDING 14 14 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 G
BINDING 22 22 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 L
end case
ACT_SITE 44 44 /note="Proton acceptor" R 1
BINDING 44 44 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 1
ACT_SITE 141 141 E 1

Additional information [?]

Size range 192-205 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]