ProRule PRU01362

General rule information [?]

Accession PRU01362
Dates 21-FEB-2023 (Created)
-- (Last updated, Version 1)
Data class Domain;
Predictors PROSITE; PS52018; DART
Name DNA ADP-ribosyl transferase (DarT) domain
Function The bacterial DNA ADP-ribosyl transferase (DarT) protein is a toxin that has been shown to transfer ADP-ribose from NAD(+) onto thymidine bases present in single-stranded DNA (ssDNA) specifically at the four-base motif TNTC, and to have no activity on RNA or protein targets.
Scope(s) Bacteria
Example(s) A0A0B0SG80 (DART_THES0);

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
CATALYTIC ACTIVITYReaction=a thymidine in DNA + NAD(+) = an N- (ADP-alpha-D-ribosyl)-thymidine in DNA + H(+) + nicotinamide; Xref=Rhea:RHEA:71651, Rhea:RHEA-COMP:13556, Rhea:RHEA-COMP:18051, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:137386, ChEBI:CHEBI:191199; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71652;
end case
SIMILARITYBelongs to the DarT ADP-ribosyltransferase family.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
case <FTGroup:1>
GO:0016779; Molecular function:nucleotidyltransferase activity
GO:0016757; Molecular function:glycosyltransferase activity

Features [?]

From: PS52018
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="DarT"
BINDING 5 7 /ligand="NAD(+)"
case <FTGroup:1>
BINDING 14 14 /ligand="NAD(+)"
BINDING 22 22 /ligand="NAD(+)"
end case
ACT_SITE 44 44 /note="Proton acceptor" R 1
BINDING 44 44 /ligand="NAD(+)"
ACT_SITE 141 141 E 1

Additional information [?]

Size range 192-205 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


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UniProtKB rule member sequences [?]