ProRule PRU01362

General rule information [?]

Accession	PRU01362
Dates	21-FEB-2023 (Created) -- (Last updated, Version 1)
Data class	Domain;
Predictors	PROSITE; PS52018; DART
Name	DNA ADP-ribosyl transferase (DarT) domain
Function	The bacterial DNA ADP-ribosyl transferase (DarT) protein is a toxin that has been shown to transfer ADP-ribose from NAD(+) onto thymidine bases present in single-stranded DNA (ssDNA) specifically at the four-base motif TNTC, and to have no activity on RNA or protein targets.
Scope(s)	Bacteria
Example(s)	A0A0B0SG80 (DART_THES0);

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
CATALYTIC ACTIVITY	Reaction=a thymidine in DNA + NAD(+) = an N- (ADP-alpha-D-ribosyl)-thymidine in DNA + H(+) + nicotinamide; Xref=Rhea:RHEA:71651, Rhea:RHEA-COMP:13556, Rhea:RHEA-COMP:18051, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:137386, ChEBI:CHEBI:191199; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71652;
end case
SIMILARITY	Belongs to the DarT ADP-ribosyltransferase family.

Keywords [?]

Glycosyltransferase
Nucleotidyltransferase
Transferase
end case
DNA-binding
Toxin-antitoxin system

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
case <FTGroup:1>
GO:0016779; Molecular function:nucleotidyltransferase activity
GO:0016757; Molecular function:glycosyltransferase activity

Features [?]

From: PS52018

Key

From

Description

Tag

Condition

FTGroup

DOMAIN

from

/note="DarT"

BINDING

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

case <FTGroup:1>

BINDING

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

BINDING

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

end case

ACT_SITE

/note="Proton acceptor"

BINDING

/ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"

ACT_SITE

141

Additional information [?]

Size range	192-205 amino acids
Related rules	None
Fusion	None
Repeats	1
Topology	Undefined

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

View rule in raw text format (no links)

UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Bacteria	[6]
All	[ 6 ]

Retrieve set of proteins with 3D structure for this domain