ProRule PRU01362
General rule information
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| PURL | https://purl.expasy.org/prosite/rule/PRU01362 |
| Accession | PRU01362 |
| Dates | 21-FEB-2023 (Created)
3-SEP-2024 (Last updated, Version 2) |
| Data class | Domain; |
| Predictors |
PROSITE; PS52018; DART |
Name | DNA ADP-ribosyl transferase (DarT) domain |
| Function | The bacterial DNA ADP-ribosyl transferase (DarT) protein is a toxin that has been shown to transfer ADP-ribose from NAD(+) onto thymidine bases present in single-stranded DNA (ssDNA) specifically at the four-base motif TNTC, and to have no activity on RNA or protein targets. |
| Scope(s) |
Bacteria |
| Example(s) | A0A0B0SG80 (DART_THES0); |
Propagated annotation
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Comments
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| case <FTGroup:1> | |
| CATALYTIC ACTIVITY | Reaction=a thymidine in DNA + NAD(+) = an N-(ADP-alpha-D-ribosyl)- thymidine in DNA + nicotinamide + H(+); Xref=Rhea:RHEA:71651, Rhea:RHEA-COMP:13556, Rhea:RHEA-COMP:18051, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:137386, ChEBI:CHEBI:191199; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71652; |
| end case | |
| SIMILARITY | Belongs to the DarT ADP-ribosyltransferase family. |
Keywords
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Gene Ontology
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| GO:0003677; Molecular function:DNA binding | |
| case <FTGroup:1> | |
| GO:0016779; Molecular function:nucleotidyltransferase activity | |
| GO:0016757; Molecular function:glycosyltransferase activity | |
Features
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| From: PS52018 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="DarT" | |||||||||
| BINDING | 5 | 7 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
1 | ||||||||
| case <FTGroup:1> | ||||||||||||
| BINDING | 14 | 14 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
G | ||||||||
| BINDING | 22 | 22 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
L | ||||||||
| end case | ||||||||||||
| ACT_SITE | 44 | 44 | /note="Proton acceptor" | R | 1 | |||||||
| BINDING | 44 | 44 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
1 | ||||||||
| ACT_SITE | 141 | 141 | E | 1 | ||||||||
Additional information
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| Size range | 192-205 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Bacteria [6] All [ 6 ]
- Retrieve set of proteins with 3D structure for this domain