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General rule information [?]

Accession PRU01366
Dates 5-MAY-2023 (Created)
5-MAY-2023 (Last updated, Version 1)
Data class Domain
Predictors PROSITE; PS52022; PV_NS1_NUC
Names Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile; Parvovirus (PV) NS1 origin of replication binding (OBD) domain; Parvovirus (PV) NS1 DNA-specific binding domain
Function The PV NS1-Nuc domain plays an important role in the "rolling hairpin" replication of the single-stranded parvoviral DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the terminal resolution site (trs). Since the DNA encountered by NS1 is double- stranded, it is assumed that binding of NS1 to its target sequences in dsDNA induces strand separation nearby, allowing for the endonuclease activity of NS1 to cleave at the trs site.

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
Cofactor Name=a divalent metal cation; Xref=CHEBI:60240;
end case

Subcellular location Host nucleus.

Gene Ontology [?]

GO:0006260; Biological process: DNA replication.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.
case <FTGroup:1>
GO:0046872; Molecular function: metal ion binding.
end case

case <FTTag:act_site>
GO:0004519; Molecular function: endonuclease activity.
end case

Keywords [?]

case <FTTag:act_site>
end case

case <FTGroup:1>
end case

Features [?]

From: PS52022
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       PV NS1-Nuc #        
MOTIF     100     102       RCR-2     H-[FWILMVACGTSYPH]-H  
MOTIF     175     179       RCR-3     Y-x(3)-K  
ACT_SITE     175     175       For nuclease activity   act_site   Y  
BINDING     93     93       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     [ED]   1
BINDING     100     100       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     H   1
BINDING     102     102       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     H   1

Additional information [?]

Size range 69-253 amino acids
Related rules None
Repeats 1
Topology Undefined
Example P07300 (NS1_MUMIM)
Comments None


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UniProtKB rule member sequences [?]