ProRule PRU01366

General rule information [?]

Accession PRU01366
Dates 5-MAY-2023 (Created)
-- (Last updated, Version 1)
Data class Domain;
Predictors PROSITE; PS52022; PV_NS1_NUC
Name Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile
Function The PV NS1-Nuc domain plays an important role in the "rolling hairpin" replication of the single-stranded parvoviral DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the terminal resolution site (trs). Since the DNA encountered by NS1 is double- stranded, it is assumed that binding of NS1 to its target sequences in dsDNA induces strand separation nearby, allowing for the endonuclease activity of NS1 to cleave at the trs site.
Scope(s) Viruses
Example(s) P07300 (NS1_MUMIM);

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
COFACTOR Name=a divalent metal cation; Xref=CHEBI:60240;
end case

Keywords [?]

Gene Ontology [?]

GO:0006260; Biological process:DNA replication
GO:0003677; Molecular function:DNA binding
GO:0042025; Cellular component:host cell nucleus
case <FTGroup:1>
GO:0046872; Molecular function:metal ion binding
end case
case <FTTag:act_site>
GO:0004519; Molecular function:endonuclease activity
end case

Features [?]

From: PS52022
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="PV NS1-Nuc #"
MOTIF 100 102 /note="RCR-2" H-[FWILMVACGTSYPH]-H
MOTIF 175 179 /note="RCR-3" Y-x(3)-K
ACT_SITE 175 175 /note="For nuclease activity" act_site Y
BINDING 93 93 /ligand="a divalent metal cation #1"
[ED] 1
BINDING 100 100 /ligand="a divalent metal cation #1"
H 1
BINDING 102 102 /ligand="a divalent metal cation #1"
H 1

Additional information [?]

Size range 69-253 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


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UniProtKB rule member sequences [?]