ProRule PRU01366
General rule information
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| PURL | https://purl.expasy.org/prosite/rule/PRU01366 |
| Accession | PRU01366 |
| Dates | 5-MAY-2023 (Created)
-- (Last updated, Version 1) |
| Data class | Domain; |
| Predictors |
PROSITE; PS52022; PV_NS1_NUC |
Name | Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile |
| Function | The PV NS1-Nuc domain plays an important role in the "rolling hairpin" replication of the single-stranded parvoviral DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the terminal resolution site (trs). Since the DNA encountered by NS1 is double- stranded, it is assumed that binding of NS1 to its target sequences in dsDNA induces strand separation nearby, allowing for the endonuclease activity of NS1 to cleave at the trs site. |
| Scope(s) |
Viruses |
| Example(s) | P07300 (NS1_MUMIM); |
Propagated annotation
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Comments
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| case <FTGroup:1> | |
| COFACTOR | Name=a divalent metal cation; Xref=CHEBI:60240; |
| end case | |
| SUBCELLULAR LOCATION | Host nucleus. |
Keywords
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| DNA-binding | |
| Host nucleus | |
| DNA replication | |
| Nucleotide-binding | |
| case <FTTag:act_site> | |
| Endonuclease | |
| Hydrolase | |
| Nuclease | |
| Covalent protein-DNA linkage | |
| end case | |
| case <FTGroup:1> | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0006260; Biological process:DNA replication | |
| GO:0003677; Molecular function:DNA binding | |
| GO:0042025; Cellular component:host cell nucleus | |
| case <FTGroup:1> | |
| GO:0046872; Molecular function:metal ion binding | |
| end case | |
| case <FTTag:act_site> | |
| GO:0004519; Molecular function:endonuclease activity | |
| end case | |
Features
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| From: PS52022 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="PV NS1-Nuc #" | |||||||||
| MOTIF | 100 | 102 | /note="RCR-2" | H-[FWILMVACGTSYPH]-H | ||||||||
| MOTIF | 175 | 179 | /note="RCR-3" | Y-x(3)-K | ||||||||
| ACT_SITE | 175 | 175 | /note="For nuclease activity" | act_site | Y | |||||||
| BINDING | 93 | 93 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240" |
[ED] | 1 | |||||||
| BINDING | 100 | 100 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240" |
H | 1 | |||||||
| BINDING | 102 | 102 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240" |
H | 1 | |||||||
Additional information
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| Size range | 69-253 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Viruses [26] All [ 26 ]
- Retrieve set of proteins with 3D structure for this domain