PRU01366

General rule information [?]

Accession	PRU01366
Dates	5-MAY-2023 (Created) 5-MAY-2023 (Last updated, Version 1)

Data class

Domain

Predictors

PROSITE; PS52022; PV_NS1_NUC

Names

Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile; Parvovirus (PV) NS1 origin of replication binding (OBD) domain; Parvovirus (PV) NS1 DNA-specific binding domain

Function

The PV NS1-Nuc domain plays an important role in the "rolling hairpin" replication of the single-stranded parvoviral DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the terminal resolution site (trs). Since the DNA encountered by NS1 is double- stranded, it is assumed that binding of NS1 to its target sequences in dsDNA induces strand separation nearby, allowing for the endonuclease activity of NS1 to cleave at the trs site.

Propagated annotation [?]

Comments [?]

case <FTGroup:1>

Cofactor

Name=a divalent metal cation; Xref=CHEBI:60240;

end case

Subcellular location

Host nucleus.

Gene Ontology [?]

GO:0006260; Biological process: DNA replication.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.

case <FTGroup:1>

GO:0046872; Molecular function: metal ion binding.

end case

case <FTTag:act_site>

GO:0004519; Molecular function: endonuclease activity.

end case

Keywords [?]

DNA-binding
Host nucleus
DNA replication
Nucleotide-binding

case <FTTag:act_site>

Endonuclease
Hydrolase
Nuclease
Covalent protein-DNA linkage

end case

case <FTGroup:1>

Metal-binding

end case

Features [?]

From: PS52022

Key From To Description Tag Condition FTGroup

DOMAIN from to PV NS1-Nuc #

MOTIF 100 102 RCR-2 H-[FWILMVACGTSYPH]-H

MOTIF 175 179 RCR-3 Y-x(3)-K

ACT_SITE 175 175 For nuclease activity act_site Y

BINDING 93 93 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 [ED] 1

BINDING 100 100 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 H 1

BINDING 102 102 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 H 1

Additional information [?]

Size range	69-253 amino acids
Related rules	None
Repeats	1
Topology	Undefined
Example	P07300 (NS1_MUMIM)
Scope	Viruses
Comments	None

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

View rule in raw text format (no links)

UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Viruses [25]
Retrieve set of proteins with 3D structure for this domain