PRU01366
General rule information
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Accession | PRU01366 |
Dates | 5-MAY-2023 (Created) 5-MAY-2023 (Last updated, Version 1) |
Data class | Domain |
Predictors | PROSITE; PS52022; PV_NS1_NUC |
Names | Parvovirus (PV) NS1 nuclease (NS1-Nuc) domain profile; Parvovirus (PV) NS1 origin of replication binding (OBD) domain; Parvovirus (PV) NS1 DNA-specific binding domain |
Function | The PV NS1-Nuc domain plays an important role in the "rolling hairpin" replication of the single-stranded parvoviral DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e., nicking) single-stranded DNA at a nearby site known as the terminal resolution site (trs). Since the DNA encountered by NS1 is double- stranded, it is assumed that binding of NS1 to its target sequences in dsDNA induces strand separation nearby, allowing for the endonuclease activity of NS1 to cleave at the trs site. |
Propagated annotation
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Comments
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case <FTGroup:1>
Cofactor | Name=a divalent metal cation; Xref=CHEBI:60240; |
end case
Subcellular location | Host nucleus. |
Gene Ontology
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GO:0006260; Biological process: DNA replication.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.
case <FTGroup:1>
GO:0046872; Molecular function: metal ion binding.
end case
case <FTTag:act_site>
GO:0004519; Molecular function: endonuclease activity.
end case
Keywords
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case <FTTag:act_site>
end case
case <FTGroup:1>
end case
Features
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From: PS52022 | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | from | to | PV NS1-Nuc # | |||||||||
MOTIF | 100 | 102 | RCR-2 | H-[FWILMVACGTSYPH]-H | ||||||||
MOTIF | 175 | 179 | RCR-3 | Y-x(3)-K | ||||||||
ACT_SITE | 175 | 175 | For nuclease activity | act_site | Y | |||||||
BINDING | 93 | 93 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 | [ED] | 1 | |||||||
BINDING | 100 | 100 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 | H | 1 | |||||||
BINDING | 102 | 102 | /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 | H | 1 |
Additional information
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Size range | 69-253 amino acids |
Related rules | None |
Repeats | 1 |
Topology | Undefined |
Example | P07300 (NS1_MUMIM) |
Scope | Viruses |
Comments | None |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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UniProtKB/Swiss-Prot sets
Viruses [25]
- Retrieve set of proteins with 3D structure for this domain