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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01378


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01378
General rule information [?]

Accession PRU01378
Dates 04-AUG-2023 (Created)
08-AUG-2023 (Last updated, Version 3)
Data class Domain;
Predictors PROSITE; PS52034; PEPTIDASE_M32
Name Peptidase family M32 domain
Function The M32 family of Zn-dependent metallocarboxypeptidases (MCPs) contains a group of hydrolases, which although being broadly distributed among prokaryotic organisms, are only present in a few eukaryotes including some green algae and trypanosomatids.
Scope(s) Bacteria
Archaea
Pyrococcus
Example(s) P50848;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTTag:act_site> and <FTGroup:1>
Protein name + AltName: EC=3.4.17.19;
end case

Comments [?]

case <FTTag:act_site> and <FTGroup:1>
CATALYTIC ACTIVITYReaction=Release of a C-terminal amino acid with broad specificity, except for -Pro.; EC=3.4.17.19;
end case
case <FTGroup:1>
COFACTOR Name=a divalent metal cation; Xref=CHEBI:60240; Note=Binds 1 divalent metal cation per subunit.
end case
SIMILARITYBelongs to the peptidase M32 family.

Keywords [?]

Carboxypeptidase
Hydrolase
Metalloprotease
Protease
end case
case <FTGroup:1>
Metal-binding
end case

Gene Ontology [?]

case <FTTag:act_site> and <FTGroup:1>
GO:0004181; Molecular function:metallocarboxypeptidase activity
GO:0006508; Biological process:proteolysis

Features [?]

From: PS52034
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase M32 #"
MOTIF 232 234 /note="HPF #" H-P-F
MOTIF 241 245 /note="DXRXT #" D-x-R-x-T
MOTIF 262 266 /note="HEXXH #" H-E-x(2)-H
MOTIF 291 294 /note="HES/GQ #" H-E-[SG]-Q
MOTIF 344 349 /note="I/NRXXA/SD #" [IN]-R-x(2)-[AS]-D
MOTIF 399 406 /note="GXXQDXHW #" G-x(2)-Q-D-x-H-W
ACT_SITE 263 263 /note="Proton donor/acceptor" act_site E
BINDING 262 262 /ligand="a divalent metal cation #"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_note="catalytic"		 H 1
BINDING 266 266 /ligand="a divalent metal cation #"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_note="catalytic"		 H 1
BINDING 292 292 /ligand="a divalent metal cation #"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_note="catalytic"		 E 1

Additional information [?]

Size range 494-504 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]