We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU01384
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01384
General rule information
[?]
| Accession | PRU01384 |
| Dates | 05-OCT-2023 (Created)
05-OCT-2023 (Last updated, Version 2) |
| Data class | Domain; |
| Predictors |
PROSITE; PS52040; TOPO_IIA |
Name | Topoisomerase (Topo) IIA-type catalytic domain |
| Function | Type IIA enzymes include topoisomerase II (topo II) encoded by both eukaryotes and viruses, and DNA gyrase and topoisomerase IV (topo IV) encoded by bacteria. The Topo IIA-type catalytic domain containing the active site tyrosine involved in the covalent complex with DNA is located on the C- terminal region of eukaryal enzymes or on the A-subunit of bacterial enzymes (GyrA or ParC for gyrase and Topo IV respectively). |
| Scope(s) |
Bacteria Eukaryota Viruses Archaea Haloferacaceae |
| Example(s) | P0C1U9; |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| case <FTTag:act_site> | |
| Protein name | + AltName: EC=5.6.2.2; |
| end case | |
Comments
[?]
| case <FTTag:act_site> | |
| CATALYTIC ACTIVITY | Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; |
| end case | |
Keywords
[?]
| Isomerase | |
| Topoisomerase | |
| end case | |
| DNA-binding | |
Gene Ontology
[?]
| GO:0003677; Molecular function:DNA binding | |
| case <FTTag:act_site> | |
| GO:0006265; Biological process:DNA topological change | |
Features
[?]
| From: PS52040 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="Topo IIA-type catalytic #" | |||||||||
| ACT_SITE | 93 | 93 | /note="O-(5'-phospho-DNA)-tyrosine intermediate" | act_site | Y | |||||||
Additional information
[?]
| Size range | 60-890 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
[?]