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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01412


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PURL: https://purl.expasy.org/prosite/rule/PRU01412
General rule information [?]

Accession PRU01412
Dates 05-JUN-2025 (Created)
05-JUN-2025 (Last updated, Version 2)
Data class Domain;
Predictors PROSITE; PS52066; MCM10_CTD
Name MCM10 C-terminal domain (MCM10-CTD)
Function The MCM10 CTD, although not present in unicellular eukaryotes, is conserved among metazoan species from nematodes to humans. The CTD contains a winged helix domain (WH) and two zinc chelating motifs, a CCCH zinc-finger (ZnF2) and a CCCC zinc-ribbon (ZnR). ssDNA binds only to ZnF2, whose structure is unique to MCM10. The ZnR is not involved in DNA binding.
Scope(s) Eukaryota
Metazoa
Example(s) Q5EAW4;

Propagated annotation [?]

Comments [?]

SIMILARITYBelongs to the MCM10 family.

Keywords [?]

Metal-binding
Zinc
Zinc-finger
end case
DNA replication
Nucleus

Gene Ontology [?]

GO:0003697; Molecular function:single-stranded DNA binding
GO:0006270; Biological process:DNA replication initiation
GO:0005634; Cellular component:nucleus
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding

Features [?]

From: PS52066
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="MCM10-CTD #"
REGION from 64 /note="Winged helix (WH) #"
case <Feature:PS52066:74=[C]> and <Feature:PS52066:77=[C]> and <Feature:PS52066:88=[C]> and <Feature:PS52066:93=[H]>
REGION 65 101 /note="CCCH-type zinc finger (ZnF2) #"
else case <FTGroup:1>
REGION 65 101 /note="CCCH-type zinc finger (ZnF2) #; atypical"
else
REGION 65 101 /note="CCCH-type zinc finger (ZnF2) #; degenerate"
end case
case <Feature:PS52066:107=[C]> and <Feature:PS52066:110=[C]> and <Feature:PS52066:125=[C]> and <Feature:PS52066:128=[C]>
REGION 102 139 /note="CCCC-type zinc ribbon (ZnR) #"
else case <FTGroup:2>
REGION 102 139 /note="CCCC-type zinc ribbon (ZnR) #; atypical"
else
REGION 102 139 /note="CCCC-type zinc ribbon (ZnR) #; degenerate"
end case
BINDING 74 74 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 77 77 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 88 88 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 93 93 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 107 107 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 [CH] 2
BINDING 110 110 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 [CH] 2
BINDING 125 125 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 [CH] 2
BINDING 128 128 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#2"		 [CH] 2

Additional information [?]

Size range 146-162 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.