We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.

ProRule PRU01419

View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01419

General rule information [?]

Accession	PRU01419
Dates	-- (Created) -- (Last updated, Version )
Data class	Domain;
Predictors	PROSITE; PS52074; COV_4ECTO
Name	Coronavirus Nsp4 ectodomain (4Ecto)
Function	Nsp4 contains six transmembranes with its ectodomain (4Ecto) localized in the intermembrane space and its carboxy-terminal domain (CTD) in the DMV lumen, forming two layers of concentric stacking hexamers constituting the lower base of the pore complex. The 3Ecto domain attaches to the upper surface of the 4Ecto domain, which is mediated by a combination of hydrophobic and hydrophilic interactions.
Scope(s)	Viruses Orthocoronavirinae
Example(s)	P0C6T5;

Propagated annotation [?]

Comments [?]

FUNCTION	[Non-structural protein 4]: Plays a role in the formation and maintenance of double membrane vesicles (DMVs) replication organelles. DMVs are formed by nsp3 and nsp4, while nsp6 zippers ER membranes and connects to lipid droplets.
SIMILARITY	Belongs to the coronaviruses polyprotein 1ab family.

Features [?]

From: PS52074

Key

From

Description

Tag

Condition

FTGroup

DOMAIN

from

/note="4Ecto #"

Additional information [?]

Size range	235-252 amino acids
Related rules	None
Fusion	None
Repeats	1
Topology	Undefined

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