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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU10127


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU10127
General rule information [?]

Accession PRU10127
Dates 16-OCT-2006 (Created)
14-MAY-2022 (Last updated, Version 11)
Data class Protein;
Predictors PROSITE; PS00171; TIM_1
PROSITE; PS51440; TIM_2
Name Triosephosphate isomerase (TIM) family
Function Triosephosphate isomerase (EC 5.3.1.1) (TIM) is the glycolytic enzyme that catalyzes the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.
Scope(s) Eukaryota
Bacteria
Archaea
Example(s) Q928I1; P34937;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and <FTGroup:2>
Identifier TPIS
Protein name + RecName: Full=Triosephosphate isomerase;
                 Short=TIM;
                 EC=5.3.1.1;
AltName: Full=Triose-phosphate isomerase;
else case <FTGroup:1>
Protein name + RecName: EC=5.3.1.-;
end case
case <FTGroup:1> and <FTGroup:2>
Gene name Name=tpiA;

Comments [?]

CATALYTIC ACTIVITY Reaction=D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate; Xref=Rhea:RHEA:18585, ChEBI:CHEBI:57642, ChEBI:CHEBI:59776; EC=5.3.1.1;
PATHWAYCarbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate from glycerone phosphate: step 1/1.
PATHWAYCarbohydrate biosynthesis; gluconeogenesis.
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
end case
case <FTGroup:1> and <FTGroup:2> and <OC:Viridiplantae>
MISCELLANEOUSIn plants, there are two types of TPIS, cytosolic and plastid.
end case
SIMILARITYBelongs to the triosephosphate isomerase family.

Keywords [?]

Isomerase
Gluconeogenesis
Glycolysis
Pentose shunt
Cytoplasm
end case

Gene Ontology [?]

case <FTGroup:1> and <FTGroup:2>
GO:0004807; Molecular function:triose-phosphate isomerase activity
GO:0006096; Biological process:glycolytic process
GO:0006094; Biological process:gluconeogenesis
GO:0006098; Biological process:pentose-phosphate shunt
GO:0005737; Cellular component:cytoplasm

Features [?]

From: PS51440
Key From To Description Tag Condition FTGroup
ACT_SITE 91 91 /note="Electrophile" H 1
ACT_SITE 162 162 /note="Proton acceptor" E 1
case <FTGroup:1>
BINDING 7 7 /ligand="substrate" N 2
BINDING 9 9 /ligand="substrate" K 2
end case
From: PS00171
Key From To Description Tag Condition FTGroup
case not <AnyFeature:PS51440>
ACT_SITE 3 3 /note="Proton acceptor"
end case

Additional information [?]

Size range 200-290 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]