 |
|
| PROSITE documentation PDOC00001 |
N-glycosylation site
Description:
It has been known for a long time [1] that potential N-glycosylation sites are
specific to the consensus sequence Asn-Xaa-Ser/Thr. It must be noted that the
presence of the consensus tripeptide is not sufficient to conclude that an
asparagine residue is glycosylated, due to the fact that the folding of the
protein plays an important role in the regulation of N-glycosylation [2]. It
has been shown [3] that the presence of proline between Asn and Ser/Thr will
inhibit N-glycosylation; this has been confirmed by a recent [4] statistical
analysis of glycosylation sites, which also shows that about 50% of the sites
that have a proline C-terminal to Ser/Thr are not glycosylated.
It must also be noted that there are a few reported cases of glycosylation
sites with the pattern Asn-Xaa-Cys; an experimentally demonstrated occurrence
of such a non-standard site is found in the plasma protein C [5].
Last update:
May 1991 / Text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| ASN_GLYCOSYLATION, PS00001; N-glycosylation site (PATTERN with a high probability of occurrence!) |
| Consensus pattern: |
N-{P}-[ST]-{P}
N is the glycosylation site |
|
|
|
References:
| 2 |
Authors | Pless D.D., Lennarz W.J. |
| Title | Enzymatic conversion of proteins to glycoproteins. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 74:134-138(1977). |
| PubMed ID | 264667 |
| 3 |
Authors | Bause E. |
| Title | Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes. |
| Source | Biochem. J. 209:331-336(1983). |
| PubMed ID | 6847620 |
| 4 |
Authors | Gavel Y., von Heijne G. |
| Title | Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. |
| Source | Protein Eng. 3:433-442(1990). |
| PubMed ID | 2349213 |
| 5 |
Authors | Miletich J.P., Broze G.J. Jr. |
| Title | Beta protein C is not glycosylated at asparagine 329. The rate of translation may influence the frequency of usage at asparagine-X-cysteine sites. |
| Source | J. Biol. Chem. 265:11397-11404(1990). |
| PubMed ID | 1694179 |
Copyright:
PROSITE is copyright. It is produced by the Swiss Institute of
Bioinformatics (SIB). There are no restrictions on its use by non-profit
institutions as long as its content is in no way modified. Usage by and
for commercial entities requires a license agreement. For information
about the licensing scheme send an email to license@isb-sib.ch or
see: http://www.expasy.org/prosite/prosite_license.htm.
Miscellaneous:
View entry in original PROSITE document format
View entry in raw text format (no links)