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| PROSITE documentation PDOC00080 |
Copper type II, ascorbate-dependent monooxygenases signatures
Description:
Copper type II, ascorbate-dependent monooxygenases [1] are a class of enzymes
that requires copper as a cofactor and which uses ascorbate as an electron
donor. The enzymes which belong to this category are:
- Dopamine-β-monooxygenase (EC 1.14.17.1) (DBH) [2], which catalyzes the
conversion of dopamine to the neurotransmitter norepinephrine.
- Peptidyl-glycine α-amidating monooxygenase (EC 1.14.17.3) (PAM), which
catalyzes the conversion of the carboxy-terminal glycine of many active
peptides and hormones to an amide group.
There are a few regions of sequence similarities between these two enzymes,
two of these regions contain clusters of conserved histidine residues which
are most probably involved in binding copper. We selected these two regions
as signature patterns.
Last update:
May 2004 / Text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| CU2_MONOOXYGENASE_1, PS00084; Copper type II, ascorbate-dependent monooxygenases signature 1 (PATTERN) |
| Consensus pattern: |
H-H-M-x(2)-F-x-C
The 2 H's are copper ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1OPM 1PHM 1SDW 1YI9 ... [ALL] |
| CU2_MONOOXYGENASE_2, PS00085; Copper type II, ascorbate-dependent monooxygenases signature 2 (PATTERN) |
| Consensus pattern: |
H-x-F-x(4)-H-T-H-x(2)-G
The 3 H's are copper ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1OPM 1PHM 1SDW 1YI9 ... [ALL] |
References:
| 1 |
Authors | Southan C., Kruse L.I. |
| Title | Sequence similarity between dopamine beta-hydroxylase and peptide alpha-amidating enzyme: evidence for a conserved catalytic domain. |
| Source | FEBS Lett. 255:116-120(1989). |
| PubMed ID | 2792366 |
| 2 |
Authors | Stewart L.C., Klinman J.P. |
| Title | Dopamine beta-hydroxylase of adrenal chromaffin granules: structure and function. |
| Source | Annu. Rev. Biochem. 57:551-592(1988). |
| PubMed ID | 3052283 |
| DOI | 10.1146/annurev.bi.57.070188.003003 |
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