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PROSITE documentation PDOC00397

Bacterial luciferase subunits signature

Description:

Luminous bacteria are abundant and widely distributed Gram-negative motile rods. The enzyme responsible for bioluminescence, bacterial luciferase [1,2,3] (EC 1.14.14.3), catalyzes the oxidation of reduced riboflavin phosphate (FMNH2) and a long chain fatty aldehyde with the emission of blue green light (490 nm). Luciferase is a heterodimeric enzyme composed of an α subunit (gene luxA) and a β subunit (gene luxB). The two subunits appear to have arisen by gene duplication.

The bioluminescence operon of some species of Photobacterium encodes a protein known as the non-fluorescent flavoprotein (NFP) (gene luxF). NFP, whose function is not yet known, contains an unusual non-covalently bound flavin. It is evolutionary related to the luxA/luxB subunits.

As a signature for this family, we selected a conserved region located in the central part of these proteins.

Last update:

June 1994 / Text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

BACTERIAL_LUCIFERASE, PS00494; Bacterial luciferase subunits signature  (PATTERN)
Consensus pattern: [GA]-[LIVM]-P-[LIVM]-x-[LIVMFY]-x-W-x(6)-[RK]-x(6)-Y-x(3)-[AR]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00494
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00494
• Scan Swiss-Prot/TrEMBL entries against PS00494
• view ligand binding statistics
Matching PDB structures: 1BRL 1BSL 1FVP 1LUC ... [ALL]

References:

1 AuthorsMeighen E.A.
TitleMolecular biology of bacterial bioluminescence.
SourceMicrobiol. Rev. 55:123-142(1991).
PubMed ID2030669
2 AuthorsMeighen E.A.
TitleBacterial bioluminescence: organization, regulation, and application of the lux genes.
SourceFASEB J. 7:1016-1022(1993).
PubMed ID8370470
3 AuthorsO'Kane D.J.O., Prasher D.C.
SourceMol. Microbiol. 6:443-449(1992).

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