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PROSITE documentation PDOC00548

Cytosol aminopeptidase signature

Description:

Cytosol aminopeptidase is a eukaryotic cytosolic zinc-dependent exopeptidase that catalyzes the removal of unsubstituted amino-acid residues from the N-terminus of proteins. This enzyme is often known as leucine aminopeptidase (EC 3.4.11.1) (LAP) but has been shown [1] to be identical with prolyl aminopeptidase (EC 3.4.11.5). Cytosol aminopeptidase is a hexamer of identical chains, each of which binds two zinc ions.

Cytosol aminopeptidase is highly similar to Escherichia coli pepA, a manganese dependent aminopeptidase. Residues involved in zinc ion-binding [2] in the mammalian enzyme are absolutely conserved in pepA where they presumably bind manganese. Most bacterial species contain a pepA-type enzyme.

As a signature pattern for these enzymes, we selected a perfectly conserved octapeptide which contains two residues involved in binding metal ions: an aspartate and a glutamate.

Note:

These proteins belong to family M17 in the classification of peptidases [3,E1].

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

CYTOSOL_AP, PS00631; Cytosol aminopeptidase signature  (PATTERN)
Consensus pattern: [NS]-[TS]-D-A-E-G-R-[LVMI]
The D and the E are zinc/manganese ligands
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00631
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00631
• Scan Swiss-Prot/TrEMBL entries against PS00631
• view ligand binding statistics
Matching PDB structures: 1BLL 1BPM 1BPN 1GYT ... [ALL]

References:

1 AuthorsMatsushima M., Takahashi T., Ichinose M., Miki K., Kurokawa K., Takahashi K.
TitleStructural and immunological evidence for the identity of prolyl aminopeptidase with leucyl aminopeptidase.
SourceBiochem. Biophys. Res. Commun. 178:1459-1464(1991).
PubMed ID1908238
2 AuthorsBurley S.K., David P.R., Sweet R.M., Taylor A., Lipscomb W.N.
TitleStructure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin.
SourceJ. Mol. Biol. 224:113-140(1992).
PubMed ID1548695
3 AuthorsRawlings N.D., Barrett A.J.
TitleEvolutionary families of metallopeptidases.
SourceMethods Enzymol. 248:183-228(1995).
PubMed ID7674922
E1
Sourcehttp://www.expasy.org/cgi-bin/lists?peptidas.txt

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