PROSITE documentation PDOC00603

S-adenosyl-L-homocysteine hydrolase signatures

Description:

S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) (AdoHcyase) is an enzyme of the activated methyl cycle, responsible for the reversible hydratation of S-adenosyl-L-homocysteine into adenosine and homocysteine. AdoHcyase is an ubiquitous enzyme which binds and requires NAD+ as a cofactor.

AdoHcyase is a highly conserved protein [1] of about 430 to 470 amino acids. As signature patterns, we selected two highly conserved regions. The first pattern is located in the N-terminal section; the second is derived from a glycine-rich region in the central part of AdoHcyase; a region thought to be involved in NAD-binding.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ADOHCYASE_1, PS00738S-adenosyl-L-homocysteine hydrolase signature 1  (PATTERN)
Consensus pattern: [GSAP]-[CS]-N-x-[FYLM]-S-[ST]-[QALKHD]-[DENG]-x-[AV]-[AVTS]-[ADERQ]-[ACSG]-[LIVMCG]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00738
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00738
Scan Swiss-Prot/TrEMBL entries against PS00738
view ligand binding statistics
Matching PDB structures: 1A7A 1B3R 1D4F 1K0U ... [ALL]
ADOHCYASE_2, PS00739S-adenosyl-L-homocysteine hydrolase signature 2  (PATTERN)
Consensus pattern: [GA]-[KSR]-x(3)-[LIV]-x-G-[FY]-G-x-[VC]-G-[KRL]-[GA]-x(1,2)-[ASC]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00739
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00739
Scan Swiss-Prot/TrEMBL entries against PS00739
view ligand binding statistics
Matching PDB structures: 1A7A 1B3R 1D4F 1K0U ... [ALL]

Reference:

1 AuthorsSganga M.W., Aksamit R.R., Cantoni G.L., Bauer C.E.
TitleMutational and nucleotide sequence analysis of S-adenosyl-L-homocysteine hydrolase from Rhodobacter capsulatus.
SourceProc. Natl. Acad. Sci. U.S.A. 89:6328-6332(1992).
PubMed ID1631127

Copyright:

PROSITE is copyright. It is produced by the Swiss Institute of Bioinformatics (SIB). There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to license@isb-sib.ch or see: http://www.expasy.org/prosite/prosite_license.htm.

Miscellaneous:

View entry in original PROSITE document format
View entry in raw text format (no links)