PROSITE documentation PDOC00643

Alpha-isopropylmalate and homocitrate synthases signatures

Description

The following enzymes have been shown [1] to be functionally as well as evolutionary related:

  • α-isopropylmalate synthase (EC 2.3.3.13) which catalyzes the first step in the biosynthesis of leucine, the condensation of acetyl-CoA and α- ketoisovalerate to form 2-isopropylmalate synthase.
  • Homocitrate synthase (EC 2.3.3.14) (gene nifV) which is involved in the biosynthesis of the iron-molybdenum cofactor of nitrogenase and catalyzes the condensation of acetyl-CoA and α-ketoglutarate into homocitrate.
  • Soybean late nodulin 56.
  • Methanococcus jannaschii hypothetical proteins MJ0503, MJ1195 and MJ1392.

We have selected two conserved regions as signature patterns for these enzymes. The first region is located in the N-terminal section while the second region is located in the central section and contains two conserved histidine residues which could be implicated in the catalytic mechanism.

Last update:

April 2006 / Pattern revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

AIPM_HOMOCIT_SYNTH_1, PS00815; Alpha-isopropylmalate and homocitrate synthases signature 1  (PATTERN)
Consensus pattern: L-R-[DE]-G-x-Q-x(4)-{L}-x(5)-K
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00815
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00815
• Scan Swiss-Prot/TrEMBL entries against PS00815
• view ligand binding statistics
Matching PDB structures: 1SR9 2ZTJ 2ZTK 2ZYF ... [ALL]
AIPM_HOMOCIT_SYNTH_2, PS00816; Alpha-isopropylmalate and homocitrate synthases signature 2  (PATTERN)
Consensus pattern: [LIVMFW]-x(2)-H-x-H-[DN]-D-x-G-x-[GAS]-x-[GASLI]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00816
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00816
• Scan Swiss-Prot/TrEMBL entries against PS00816
• view ligand binding statistics
Matching PDB structures: 1SR9 2ZTJ 2ZTK 2ZYF ... [ALL]

Reference

1 Authors Wang S.-Z., Dean D.R., Chen J.S., Johnson J.L.
Title The N-terminal and C-terminal portions of NifV are encoded by two different genes in Clostridium pasteurianum.
Source J. Bacteriol. 173:3041-3046(1991).
PubMed ID 2022611

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