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| PROSITE documentation PDOC00657 |
Aspartyl proteases, omptin family signatures
Description:
Escherichia coli protease VII (EC 3.4.21.87) (omptin) (gene ompT) [1] is an
outer-membrane associated protease with a specificity for paired basic
residues. It seems to be an aspartyl protease [2] inhibited by zinc ions.
Omptin is highly similar to the following proteins:
- Salmonella typhimurium outer membrane protease E (gene pgtE) [3], also
specific for paired basic residues.
- Yersinia pestis coagulase/fibrinolysin (plasminogen activator) (gene pla)
[4]. This enzyme is encoded on a plasmid required for the pathogenic
potential of the bacteria. It cleaves and activates plasminogen at the foci
of infection; this allows plasmin to degrade fibrin barriers thus promoting
the invasiveness of the plague bacteria.
- Escherichia coli outer membrane protease ompP [5].
The mature forms of these enzymes are proteins of about 280 to 300 amino-acid
residues. As signature patterns, we selected two well conserved regions in the
central part of these proteins.
Note:
These proteins belong to family A26 in the classification of peptidases
[6,E1].
Last update:
January 2002 / Text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| OMPTIN_1, PS00834; Aspartyl proteases, omptin family signature 1 (PATTERN) |
| Consensus pattern: |
W-T-D-x-S-x-H-P-x-T
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
2X4M 2X55 2X56 [ALL] |
| OMPTIN_2, PS00835; Aspartyl proteases, omptin family signature 2 (PATTERN) |
| Consensus pattern: |
A-G-Y-Q-E-[ST]-R-[FYW]-S-[FYW]-[TN]-A-x-G-G-[ST]-Y
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1I78 2X4M 2X55 2X56 [ALL] |
References:
| 1 |
Authors | Sugimura K., Nishihara T. |
| Title | Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. |
| Source | J. Bacteriol. 170:5625-5632(1988). |
| PubMed ID | 3056908 |
| 2 |
Authors | Vandeputte-Rutten L., Kramer R.A., Kroon J., Dekker N., Egmond M.R., Gros P. |
| Source | EMBO J. 20:5033-5039(2001). |
| 3 |
Authors | Grodberg J., Dunn J.J. |
| Title | Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein. |
| Source | J. Bacteriol. 171:2903-2905(1989). |
| PubMed ID | 2651422 |
| 4 |
Authors | Sodeinde O.A., Goguen J.D. |
| Title | Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of Salmonella typhimurium. |
| Source | Infect. Immun. 57:1517-1523(1989). |
| PubMed ID | 2651310 |
| 5 |
Authors | Kaufmann A., Stierhof Y.-D., Henning U. |
| Title | New outer membrane-associated protease of Escherichia coli K-12. |
| Source | J. Bacteriol. 176:359-367(1994). |
| PubMed ID | 8288530 |
| 6 |
Authors | Rawlings N.D., Barrett A.J. |
| Title | Families of serine peptidases. |
| Source | Methods Enzymol. 244:19-61(1994). |
| PubMed ID | 7845208 |
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