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PROSITE documentation PDOC00669

Signal peptidases II signature

Description:

Signal peptidases (SPases) [1] (also known as leader peptidases) remove the signal peptides from secretory proteins. In prokaryotes three types of SPases are known: type I (gene lepB) which is responsible for the processing of the majority of exported pre-proteins; type II (gene lsp) which only process lipoproteins, and a third type involved in the processing of pili subunits.

SPase II (EC 3.4.23.36), also known as lipoprotein signal peptidase, recognizes a conserved sequence and cuts in front of a cysteine residue to which a glyceride-fatty acid lipid is attached. SPase II is an integral membrane protein that is anchored in the cytoplasmic membrane.

There are a number of conserved regions in the sequence of Spase II from various bacteria [2], we have selected one of these regions as a signature pattern.

Note:

These proteins belong to family A8 in the classification of peptidases [3,E1].

Expert(s) to contact by email:

von Heijne G.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

SPASE_II, PS00855; Signal peptidases II signature  (PATTERN)
Consensus pattern: [LIVM]-x-[GASF]-[GA]-[GAST]-[LIVMTF]-[GAS]-N-[LVMFGIA]-[LIVFYGT]-D-[RIK]-[LIVMFAC]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00855
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00855
• Scan Swiss-Prot/TrEMBL entries against PS00855
• view ligand binding statistics

References:

1 AuthorsDalbey R.E., Von Heijne G.
TitleSignal peptidases in prokaryotes and eukaryotes--a new protease family.
SourceTrends Biochem. Sci. 17:474-478(1992).
PubMed ID1455520
2 AuthorsZhao X.J., Wu H.C.
TitleNucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) gene.
SourceFEBS Lett. 299:80-84(1992).
PubMed ID1544479
3 AuthorsRawlings N.D., Barrett A.J.
TitleEvolutionary families of metallopeptidases.
SourceMethods Enzymol. 248:183-228(1995).
PubMed ID7674922
E1
Sourcehttp://www.expasy.org/cgi-bin/lists?peptidas.txt

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