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GTP cyclohydrolase I (EC 3.5.4.16) catalyzes the biosynthesis of formic acid and dihydroneopterin triphosphate from GTP. This reaction is the first step in the biosynthesis of tetrahydrofolate in prokaryotes, of tetrahydrobiopterin in vertebrates, and of pteridine-containing pigments in insects.

GTP cyclohydrolase I is a protein of from 190 to 250 amino acid residues. The comparison of the sequence of the enzyme from bacterial and eukaryotic sources shows that the structure of this enzyme has been extremely well conserved throughout evolution [1].

As signature patterns we selected two conserved regions. The first contains a perfectly conserved tetrapeptide which is part of the GTP-binding pocket [2], the second region also contains conserved residues involved in GTP-binding.

April 2006 / Pattern revised.

PROSITE methods (with tools and information) covered by this documentation:

GTP_CYCLOHYDROL_1_1, PS00859; GTP cyclohydrolase I signature 1  (PATTERN)

Consensus pattern: [DENGQST]-[LIVMPF]-[LIVM]-x(1,2)-[KRNQELD]-[DENKGS]-[LIVM]-x(3)-[STG]-x-C-[EP]-H(2) Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00859 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00859 • Scan Swiss-Prot/TrEMBL entries against PS00859 • view ligand binding statistics

Matching PDB structures: 1FB1 1FBX 1GTP 1IS7 ... [ALL]

GTP_CYCLOHYDROL_1_2, PS00860; GTP cyclohydrolase I signature 2  (PATTERN)

Consensus pattern: [SA]-x-[RK]-x-Q-[LIVMT]-Q-E-[RNAK]-[LIM]-[TSNV] Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00860 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00860 • Scan Swiss-Prot/TrEMBL entries against PS00860 • view ligand binding statistics

Matching PDB structures: 1A8R 1A9C 1FB1 1FBX ... [ALL]

1	Authors	Maier J., Witter K., Gutlich M., Ziegler I., Werner T., Ninnemann H.
	Title	Homology cloning of GTP-cyclohydrolase I from various unrelated eukaryotes by reverse-transcription polymerase chain reaction using a general set of degenerate primers.
	Source	Biochem. Biophys. Res. Commun. 212:705-711(1995).
	PubMed ID	7542887

2	Authors	Nar H., Huber R., Meining W., Schmid C., Weinkauf S., Bacher A.
	Title	Atomic structure of GTP cyclohydrolase I.
	Source	Structure 3:459-466(1995).
	PubMed ID	7663943

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