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PROSITE documentation PDOC00690

Dihydroxy-acid and 6-phosphogluconate dehydratases signatures

Description:

Two dehydratases have been shown [1] to be evolutionary related:

  • Dihydroxy-acid dehydratase (EC 4.2.1.9) (gene ilvD or ILV3) which catalyzes the fourth step in the biosynthesis of isoleucine and valine, the dehydratation of 2,3-dihydroxy-isovaleic acid into α-ketoisovaleric acid.
  • 6-phosphogluconate dehydratase (EC 4.2.1.12) (gene edd) which catalyzes the first step in the Entner-Doudoroff pathway, the dehydratation of 6-phospho- D-gluconate into 6-phospho-2-dehydro-3-deoxy-D-gluconate.
  • Escherichia coli hypothetical protein yjhG.

Both enzymes are proteins of about 600 amino acid residues. We selected two highly conserved regions as signature patterns. The first pattern is located in the N-terminal part and contains a cysteine that could be involved in the binding of a 2Fe-2S iron-sulfur cluster [2]. The second pattern is located in the C-terminal half.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ILVD_EDD_1, PS00886; Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1  (PATTERN)
Consensus pattern: C-D-K-x(2)-P-[GA]-x(3)-[GA]
The C could be a 2Fe-2S ligand
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00886
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00886
• Scan Swiss-Prot/TrEMBL entries against PS00886
• view ligand binding statistics
ILVD_EDD_2, PS00887; Dihydroxy-acid and 6-phosphogluconate dehydratases signature 2  (PATTERN)
Consensus pattern: [SGALC]-[LIMF]-[LIVMF]-T-D-[GA]-R-[LIVMFY]-S-[GA]-[GAV]-[ST]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00887
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00887
• Scan Swiss-Prot/TrEMBL entries against PS00887
• view ligand binding statistics

References:

1 AuthorsEgan S.E., Fliege R., Tong S., Shibata A., Wolf R.E. Jr., Conway T.
TitleMolecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon.
SourceJ. Bacteriol. 174:4638-4646(1992).
PubMed ID1624451
2 AuthorsVelasco J.A., Cansado J., Pena M.C., Kawakami T., Laborda J., Notario V.
TitleCloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae.
SourceGene 137:179-185(1993).
PubMed ID8299945

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