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Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) is a FMN flavoprotein involved in the de novo synthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. It oxidizes pyridoxamine-5-P (PMP) and pyridoxine-5-P (PNP) to pyridoxal-5-P. The sequences of the enzyme from bacterial (genes pdxH or fprA) [1] and fungal (gene PDX3) [2] sources show that this protein has been highly conserved throughout evolution.

PdxH is evolutionary related [3] to one of the enzymes in the phenazine biosynthesis protein pathway, phzD (also known as phzG).

As a signature pattern, we selected a highly conserved region located in the C-terminal part of these enzymes.

April 2006 / Pattern revised.

PROSITE method (with tools and information) covered by this documentation:

PYRIDOX_OXIDASE, PS01064; Pyridoxamine 5'-phosphate oxidase signature  (PATTERN)

Consensus pattern: [LIVFMYWA]-E-F-W-[QHGENRAMVYLCF]-x(4)-[RW]-[LIVMK]-[HN]-[DNESIT]-R Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01064 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01064 • Scan Swiss-Prot/TrEMBL entries against PS01064 • view ligand binding statistics

Matching PDB structures: 1CI0 1DNL 1G76 1G77 ... [ALL]

1	Authors	Lam H.-M., Winkler M.E.
	Title	Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations.
	Source	J. Bacteriol. 174:6033-6045(1992).
	PubMed ID	1356963

2	Authors	Loubbardi A., Marcireau C., Karst F., Guilloton M.
	Title	Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase.
	Source	J. Bacteriol. 177:1817-1823(1995).
	PubMed ID	7896706

3	Authors	Pierson L.S. III, Gaffney T., Lam S., Gong F.
	Title	Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84.
	Source	FEMS Microbiol. Lett. 134:299-307(1995).
	PubMed ID	8586283

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