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PROSITE documentation PDOC01036

Pyrrolidone-carboxylate peptidase active sites

Description:

Pyrrolidone-carboxylate peptidase (EC 3.4.19.3) (PYRase) (also known as pyroglutamyl peptidase) is the enzyme that selectively removes pyroglutamate (pGlu) from the N-terminus of proteins and peptides.

In bacteria and archebacteria PYRase (gene pcp) is a protein of 22-25 kD. It is a cysteine protease with a Cys-His-Glu catalytic triad [1,2]. We developed two signature patterns that respectively include the glutamate and cysteine active site residues.

Note:

These proteins belong to family C15 in the classification of peptidases [3,E1].

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

PYRASE_CYS, PS01334; Pyrrolidone-carboxylate peptidase cysteine active site  (PATTERN)
Consensus pattern: [LIVF]-x-[GSAVC]-x-[LIVM]-S-x-[STAD]-A-G-x-[FY]-[LIVN]-C-[DNS]
C is the active site residue
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01334
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01334
• Scan Swiss-Prot/TrEMBL entries against PS01334
• view ligand binding statistics
Matching PDB structures: 1A2Z 1AUG 1IOF 1IU8 ... [ALL]
PYRASE_GLU, PS01333; Pyrrolidone-carboxylate peptidase glutamic acid active site  (PATTERN)
Consensus pattern: G-x(2)-[GAP]-x(4)-[LIV]-[ST]-x-E-[KR]-[LIVC]-[AG]-x-[NG]
E is the active site residue
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01333
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01333
• Scan Swiss-Prot/TrEMBL entries against PS01333
• view ligand binding statistics
Matching PDB structures: 1A2Z 1AUG 1IOF 1IOI ... [ALL]

References:

1 AuthorsSingleton M.R., Isupov M., Littlechild J.
TitleX-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis.
SourceStructure 7:237-244(1999).
PubMed ID10368293
2 AuthorsOdagaki Y., Hayashi A., Okada K., Hirotsu K., Kabashima T., Ito K., Yoshimoto T., Tsuru D., Sato M., Clardy J.
TitleThe crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease.
SourceStructure 7:399-411(1999).
PubMed ID10196127
3 AuthorsRawlings N.D., Barrett A.J.
TitleFamilies of cysteine peptidases.
SourceMethods Enzymol. 244:461-486(1994).
PubMed ID7845226
E1
Sourcehttp://www.expasy.org/cgi-bin/lists?peptidas.txt

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