PROSITE documentation PDOC00071Glu / Leu / Phe / Val dehydrogenases active site
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Description
- Glutamate dehydrogenases (EC 1.4.1.2, EC 1.4.1.3, and EC 1.4.1.4) (GluDH) are enzymes that catalyze the NAD- or NADP-dependent reversible deamination of glutamate into α-ketoglutarate [1,2]. GluDH isozymes are generally involved with either ammonia assimilation or glutamate catabolism.
- Leucine dehydrogenase (EC 1.4.1.9) (LeuDH) is a NAD-dependent enzyme that catalyzes the reversible deamination of leucine and several other aliphatic amino acids to their keto analogues [3].
- Phenylalanine dehydrogenase (EC 1.4.1.20) (PheDH) is a NAD-dependent enzyme that catalyzes the reversible deamidation of L-phenylalanine into phenyl- pyruvate [4].
- Valine dehydrogenase (EC 1.4.1.8) (ValDH) is a NADP-dependent enzyme that catalyzes the reversible deamidation of L-valine into 3-methyl-2- oxobutanoate [5].
These dehydrogenases are structurally and functionally related. A conserved lysine residue located in a glycine-rich region has been implicated in the catalytic mechanism. The conservation of the region around this residue allows the derivation of a signature pattern for such type of enzymes.
Note:All known sequences from this family have Pro in the last position of the pattern with the exception of yeast GluDH which as Leu.
Last update:November 1997 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Britton K.L. Baker P.J. Rice D.W. Stillman T.J. |
| Title | Structural relationship between the hexameric and tetrameric family of glutamate dehydrogenases. | |
| Source | Eur. J. Biochem. 209:851-859(1992). | |
| PubMed ID | 1358610 |
| 2 | Authors | Benachenhou-Lahfa N. Forterre P. Labedan B. |
| Source | J. Mol. Evol. 36:335-346(1993). |
| 3 | Authors | Nagata S. Tanizawa K. Esaki N. Sakamoto Y. Ohshima T. Tanaka H. Soda K. |
| Title | Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases. | |
| Source | Biochemistry 27:9056-9062(1988). | |
| PubMed ID | 3069133 |
| 4 | Authors | Takada H. Yoshimura T. Ohshima T. Esaki N. Soda K. |
| Title | Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene. | |
| Source | J. Biochem. 109:371-376(1991). | |
| PubMed ID | 1880121 |
| 5 | Authors | Tang L. Hutchinson C.R. |
| Title | Sequence, transcriptional, and functional analyses of the valine (branched-chain amino acid) dehydrogenase gene of Streptomyces coelicolor. | |
| Source | J. Bacteriol. 175:4176-4185(1993). | |
| PubMed ID | 8320231 |
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