We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00071Glu / Leu / Phe / Val dehydrogenases active site
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00071
Description
- Glutamate dehydrogenases (EC 1.4.1.2, EC 1.4.1.3, and EC 1.4.1.4) (GluDH) are enzymes that catalyze the NAD- or NADP-dependent reversible deamination of glutamate into α-ketoglutarate [1,2]. GluDH isozymes are generally involved with either ammonia assimilation or glutamate catabolism.
- Leucine dehydrogenase (EC 1.4.1.9) (LeuDH) is a NAD-dependent enzyme that catalyzes the reversible deamination of leucine and several other aliphatic amino acids to their keto analogues [3].
- Phenylalanine dehydrogenase (EC 1.4.1.20) (PheDH) is a NAD-dependent enzyme that catalyzes the reversible deamidation of L-phenylalanine into phenyl- pyruvate [4].
- Valine dehydrogenase (EC 1.4.1.8) (ValDH) is a NADP-dependent enzyme that catalyzes the reversible deamidation of L-valine into 3-methyl-2- oxobutanoate [5].
These dehydrogenases are structurally and functionally related. A conserved lysine residue located in a glycine-rich region has been implicated in the catalytic mechanism. The conservation of the region around this residue allows the derivation of a signature pattern for such type of enzymes.
Note:All known sequences from this family have Pro in the last position of the pattern with the exception of yeast GluDH which as Leu.
Last update:November 1997 / Pattern and text revised.
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Technical section
PROSITE method (with tools and information) covered by this documentation:
References
| 1 | Authors | Britton K.L. Baker P.J. Rice D.W. Stillman T.J. |
| Title | Structural relationship between the hexameric and tetrameric family of glutamate dehydrogenases. | |
| Source | Eur. J. Biochem. 209:851-859(1992). | |
| PubMed ID | 1358610 |
| 2 | Authors | Benachenhou-Lahfa N. Forterre P. Labedan B. |
| Source | J. Mol. Evol. 36:335-346(1993). |
| 3 | Authors | Nagata S. Tanizawa K. Esaki N. Sakamoto Y. Ohshima T. Tanaka H. Soda K. |
| Title | Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases. | |
| Source | Biochemistry 27:9056-9062(1988). | |
| PubMed ID | 3069133 |
| 4 | Authors | Takada H. Yoshimura T. Ohshima T. Esaki N. Soda K. |
| Title | Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene. | |
| Source | J. Biochem. 109:371-376(1991). | |
| PubMed ID | 1880121 |
| 5 | Authors | Tang L. Hutchinson C.R. |
| Title | Sequence, transcriptional, and functional analyses of the valine (branched-chain amino acid) dehydrogenase gene of Streptomyces coelicolor. | |
| Source | J. Bacteriol. 175:4176-4185(1993). | |
| PubMed ID | 8320231 |
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