PROSITE documentation PDOC00381HSF-type DNA-binding domain signature
Heat shock factor (HSF) is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). HSE is a palindromic element rich with repetitive purine and pyrimidine motifs: 5'-nGAAnnTTCnnGAAnnTTCn-3'. HSF is expressed at normal temperatures but is activated by heat shock or chemical stressors [1,2]. The sequences of HSF from various species show extensive similarity in a region of about 90 amino acids, which has been shown [3] to bind DNA.
Some other proteins also contain a HSF domain, these are:
- Yeast SFL1, a protein involved in cell surface assembly and regulation of the gene related to flocculation (asexual cell aggregation) [4].
- Yeast transcription factor SKN7 (or BRY1 or POS9), which binds to the promoter elements SCB and MCB essential for the controal of G1 cyclins expression [5].
- Yeast MGA1.
- Yeast hypothetical protein YJR147w.
We have derived a pattern from the most conserved part of the HSF DNA-binding domain, its central region.
Last update:November 1997 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Sorger P.K. |
| Title | Heat shock factor and the heat shock response. | |
| Source | Cell 65:363-366(1991). | |
| PubMed ID | 2018972 |
| 2 | Authors | Mager W.H. Moradas Ferreira P.M. |
| Title | Stress response of yeast. | |
| Source | Biochem. J. 290:1-13(1993). | |
| PubMed ID | 8439279 |
| 3 | Authors | Vuister G.W. Kim S.-J. Orosz A. Marquardt J. Wu C. Bax A. |
| Title | Solution structure of the DNA-binding domain of Drosophila heat shock transcription factor. | |
| Source | Nat. Struct. Biol. 1:605-614(1994). | |
| PubMed ID | 7634100 |
| 4 | Authors | Fujita A. Kikuchi Y. Kuhara S. Misumi Y. Matsumoto S. Kobayashi H. |
| Title | Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor. | |
| Source | Gene 85:321-328(1989). | |
| PubMed ID | 2697640 |
| 5 | Authors | Morgan B.A. Bouquin N. Merrill G.F. Johnston L.H. |
| Title | A yeast transcription factor bypassing the requirement for SBF and DSC1/MBF in budding yeast has homology to bacterial signal transduction proteins. | |
| Source | EMBO J. 14:5679-5689(1995). | |
| PubMed ID | 8521825 |
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