Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00381HSF-type DNA-binding domain signature
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC00381
Heat shock factor (HSF) is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). HSE is a palindromic element rich with repetitive purine and pyrimidine motifs: 5'-nGAAnnTTCnnGAAnnTTCn-3'. HSF is expressed at normal temperatures but is activated by heat shock or chemical stressors [1,2]. The sequences of HSF from various species show extensive similarity in a region of about 90 amino acids, which has been shown [3] to bind DNA.
Some other proteins also contain a HSF domain, these are:
- Yeast SFL1, a protein involved in cell surface assembly and regulation of the gene related to flocculation (asexual cell aggregation) [4].
- Yeast transcription factor SKN7 (or BRY1 or POS9), which binds to the promoter elements SCB and MCB essential for the controal of G1 cyclins expression [5].
- Yeast MGA1.
- Yeast hypothetical protein YJR147w.
We have derived a pattern from the most conserved part of the HSF DNA-binding domain, its central region.
Last update:November 1997 / Pattern and text revised.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Sorger P.K. |
| Title | Heat shock factor and the heat shock response. | |
| Source | Cell 65:363-366(1991). | |
| PubMed ID | 2018972 |
| 2 | Authors | Mager W.H. Moradas Ferreira P.M. |
| Title | Stress response of yeast. | |
| Source | Biochem. J. 290:1-13(1993). | |
| PubMed ID | 8439279 |
| 3 | Authors | Vuister G.W. Kim S.-J. Orosz A. Marquardt J. Wu C. Bax A. |
| Title | Solution structure of the DNA-binding domain of Drosophila heat shock transcription factor. | |
| Source | Nat. Struct. Biol. 1:605-614(1994). | |
| PubMed ID | 7634100 |
| 4 | Authors | Fujita A. Kikuchi Y. Kuhara S. Misumi Y. Matsumoto S. Kobayashi H. |
| Title | Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor. | |
| Source | Gene 85:321-328(1989). | |
| PubMed ID | 2697640 |
| 5 | Authors | Morgan B.A. Bouquin N. Merrill G.F. Johnston L.H. |
| Title | A yeast transcription factor bypassing the requirement for SBF and DSC1/MBF in budding yeast has homology to bacterial signal transduction proteins. | |
| Source | EMBO J. 14:5679-5689(1995). | |
| PubMed ID | 8521825 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.