PROSITE documentation PDOC00411RNA polymerases D / 30 to 40 Kd subunits signature
In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.
It has been shown [1,2,3] that small subunits of about 30 to 40 Kd found in all three types of eukaryotic polymerases are highly conserved. Subunits known to belong to this family are:
- Budding yeast RPC5 subunit (or RPC40) from RNA polymerases I and III.
- Mammalian RPA40 from RNA polymerase I.
- Budding yeast RPB3 subunit from RNA polymerase II.
- Fission yeast rpb3 subunit from RNA polymerase II.
- Mammalian RPB3 (or RPB33) (gene POLR2C) from RNA polymerase II.
- Conjugation stage-specific protein cnjC from Tetrahymena thermophila, which may be a stage-specific RNA polymerase subunit.
- Archebacterial RNA polymerase subunit D (gene rpoD).
As a signature pattern for these subunits, we selected a conserved region in their N-terminal part.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Kolodziej P. Young R.A. |
| Title | RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. | |
| Source | Mol. Cell. Biol. 9:5387-5394(1989). | |
| PubMed ID | 2685562 |
| 2 | Authors | Pati U.K. Weissman S.M. |
| Title | The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes. | |
| Source | J. Biol. Chem. 265:8400-8403(1990). | |
| PubMed ID | 2187864 |
| 3 | Authors | Azuma Y. Yamagishi M. Ishihama A. |
| Title | Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene. | |
| Source | Nucleic Acids Res. 21:3749-3754(1993). | |
| PubMed ID | 8367291 |
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