PROSITE documentation PDOC00447Alkylbase DNA glycosidases alkA family signature
Alkylbase DNA glycosidases [1] are DNA repair enzymes that hydrolyzes the deoxyribose N-glycosidic bond to excise various alkylated bases from a damaged DNA polymer.
In Escherichia coli there are two alkylbase DNA glycosidases: one (gene tag) which is constitutively expressed and which is specific for the removal of 3-methyladenine (EC 3.2.2.20), and one (gene alkA) which is induced during adaptation to alkylation and which can remove a variety of alkylation products (EC 3.2.2.21). Tag and alkA do not share any region of sequence similarity.
In yeast there is an alkylbase DNA glycosidase (gene MAG1) [2,3], which can remove 3-methyladenine or 7-methyladenine and which is structurally related to alkA.
MAG and alkA are both proteins of about 300 amino acid residues. While the C- and N-terminal ends appear to be unrelated, there is a central region of about 130 residues which is well conserved. As a signature pattern we have selected part of this region.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Lindahl T. Sedgwick B. Sekiguchi M. Nakabeppu Y. |
| Title | Regulation and expression of the adaptive response to alkylating agents. | |
| Source | Annu. Rev. Biochem. 57:133-157(1988). | |
| PubMed ID | 3052269 | |
| DOI | 10.1146/annurev.bi.57.070188.001025 |
| 2 | Authors | Berdal K.G. Bjoras M. Bjelland S. Seeberg E.C. |
| Title | Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene. | |
| Source | EMBO J. 9:4563-4568(1990). | |
| PubMed ID | 2265619 |
| 3 | Authors | Chen J. Derfler B. Samson L. |
| Title | Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage. | |
| Source | EMBO J. 9:4569-4575(1990). | |
| PubMed ID | 2265620 |
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