PROSITE documentation PDOC00483Eukaryotic mitochondrial porin signature
The major protein of the outer mitochondrial membrane of eukaryotes is a porin that forms a voltage-dependent anion-selective channel (VDAC) that behaves as a general diffusion pore for small hydrophilic molecules [1,2,3,4]. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV.
This protein contains about 280 amino acids and its sequence is composed of between 12 to 16 β-strands that span the mitochondrial outer membrane. Yeast contains two members of this family (genes POR1 and POR2); vertebrates have at least three members (genes VDAC1, VDAC2 and VDAC3) [5].
As a signature pattern we selected a conserved region located at the C-terminal part of these proteins.
Last update:July 1999 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Benz R. |
| Title | Permeation of hydrophilic solutes through mitochondrial outer membranes: review on mitochondrial porins. | |
| Source | Biochim. Biophys. Acta 1197:167-196(1994). | |
| PubMed ID | 8031826 |
| 2 | Authors | Manella C.A. |
| Source | Trends Biochem. Sci. 17:315-320(1992). |
| 3 | Authors | Dihanich M. |
| Title | The biogenesis and function of eukaryotic porins. | |
| Source | Experientia 46:146-153(1990). | |
| PubMed ID | 1689252 |
| 4 | Authors | Forte M. Guy H.R. Mannella C.A. |
| Title | Molecular genetics of the VDAC ion channel: structural model and sequence analysis. | |
| Source | J. Bioenerg. Biomembr. 19:341-350(1987). | |
| PubMed ID | 2442148 |
| 5 | Authors | Sampson M.J. Lovell R.S. Davison D.B. Craigen W.J. |
| Title | A novel mouse mitochondrial voltage-dependent anion channel gene localizes to chromosome 8. | |
| Source | Genomics 36:192-196(1996). | |
| PubMed ID | 8812436 |
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