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PROSITE documentation PDOC00725
Gamma-thionins family signature

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PURL: https://purl.expasy.org/prosite/documentation/PDOC00725

Description

The following small plant proteins are evolutionary related:

γ-thionins from wheat endosperm (γ-purothionins) and barley (γ- hordothionins) which are toxic to animal cells and inhibit protein synthesis in cell free systems [1].
A flower-specific thionin (FST) from tobacco [2].
Antifungal proteins (AFP) from the seeds of Brassicaceae species such as radish, mustard, turnip and Arabidopsis thaliana [3].
Inhibitors of insect α-amylases from sorghum [4].
Probable protease inhibitor P322 from potato.
A germination-related protein from cowpea [5].
Anther-specific protein SF18 from sunflower [6]. SF18 is a protein that contains a γ-thionin domain at its N-terminus and a proline-rich C- terminal domain.
Soybean sulfur-rich protein SE60 [7].
Vicia faba antibacterial peptides fabatin-1 and -2.

In their mature form, these proteins generally consist of about 45 to 50 amino-acid residues. As shown in the following schematic representation, these peptides contain eight conserved cysteines involved in disulfide bonds.

          +-------------------------------------------+
          |          +-------------------+            |
          |          |                   |            |
        xxCxxxxxxxxxxCxxxxxCxxxCxxxxxxxxxCxxxxxxCxCxxxC
        *******************|***|                | |
                           +---|----------------+ |
                               +------------------+

'C': conserved cysteine involved in a disulfide bond.
'*': position of the pattern.

Last update:

December 2004 / Pattern and text revised.

-------------------------------------------------------------------------------

Technical section

PROSITE method (with tools and information) covered by this documentation:

GAMMA_THIONIN, PS00940; Gamma-thionins family signature (PATTERN)

Consensus pattern:
[KRGAQS]-x-C-x(3)-[SVA]-x(2)-[FYWH]-x(1,2)-[GF]-x-C-x(5)-C-x(3)-C
The 4 C's are involved in disulfide bonds
Sequences in UniProtKB/Swiss-Prot known to belong to this class: 90
- detected by PS00940: 80 (true positives)
- undetected by PS00940: 10 (7 false negatives and 3 'partials')
Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00940:
NONE.
Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
Clustal format, color, condensed view / Clustal format, color / Clustal format, plain text / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00940
Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00940
Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00940
Matching PDB structures: pdb_00001ayj pdb_00001gps pdb_00001gpt pdb_00001jkz ... [ALL]

References

1	Authors	Bruix M. Jimenez M.A. Santoro J. Gonzalez C. Colilla F.J. Mendez E. Rico M.
	Title	Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins.
	Source	Biochemistry 32:715-724(1993).
	PubMed ID	8380707

2	Authors	Gu Q. Kawata E.E. Morse M.-J. Wu H.-M. Cheung A.Y.
	Title	A flower-specific cDNA encoding a novel thionin in tobacco.
	Source	Mol. Gen. Genet. 234:89-96(1992).
	PubMed ID	1495489

3	Authors	Terras F.R.G. Torrekens S. van Leuven F. Osborn R.W. Vanderleyden J. Cammue B.P.A. Broekaert W.F.
	Title	A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.
	Source	FEBS Lett. 316:233-240(1993).
	PubMed ID	8422949

4	Authors	Bloch C. Jr. Richardson M.
	Title	A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies with wheat gamma-purothionins.
	Source	FEBS Lett. 279:101-104(1991).
	PubMed ID	1995329

5	Authors	Ishibashi N. Yamauchi D. Miniamikawa T.
	Title	Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination.
	Source	Plant Mol. Biol. 15:59-64(1990).
	PubMed ID	2103443

6	Authors	Domon C. Evrard J.L. Herdenberger F. Pillay D.T. Steinmetz A.
	Title	Nucleotide sequence of two anther-specific cDNAs from sunflower (Helianthus annuus L.).
	Source	Plant Mol. Biol. 15:643-646(1990).
	PubMed ID	2102380

7	Authors	Choi Y. Choi Y.D. Lee J.S.
	Title	Nucleotide sequence of a cDNA encoding a low molecular weight sulfur-rich protein in soybean seeds.
	Source	Plant Physiol. 101:699-700(1993).
	PubMed ID	8278516
	DOI	101/2/699

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PROSITE documentation PDOC00725Gamma-thionins family signature

PROSITE documentation PDOC00725
Gamma-thionins family signature