PROSITE documentation PDOC00854RNA polymerases H / 23 Kd subunits signature
In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.
Archaebacterial subunit H (gene rpoH) [1,2] is a small protein of about 8.5 to 10 Kd, it is evolutionary related to the C-terminal part of a 23 Kd component shared by all three forms of eukaryotic RNA polymerases (gene RPB5 in yeast and POLR2E in mammals).
As a signature pattern we selected a conserved region which is located at the N-terminal extremity of subunit H; this region contains two histidines that could play a role in the binding of a metal ion.
Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Klenk H.-P. Palm P. Lottspeich F. Zillig W. |
| Title | Component H of the DNA-dependent RNA polymerases of Archaea is homologous to a subunit shared by the three eucaryal nuclear RNA polymerases. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 89:407-410(1992). | |
| PubMed ID | 1729711 |
| 2 | Authors | Thiru A. Hodach M. Eloranta J.J. Kostourou V. Weinzierl R.O. Matthews S. |
| Title | RNA polymerase subunit H features a beta-ribbon motif within a novel fold that is present in archaea and eukaryotes. | |
| Source | J. Mol. Biol. 287:753-760(1999). | |
| PubMed ID | 10191143 |
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