PROSITE documentation PDOC00904Ribonuclease II family signature
Description
On the basis of sequence similarities, the following bacterial and eukaryotic proteins seem to form a family:
- Escherichia coli and related bacteria ribonuclease II (EC 3.1.13.1) (RNase II) (gene rnb) [1]. RNase II is an exonuclease involved in mRNA decay. It degrades mRNA by hydrolyzing single-stranded polyribonucleotides processively in the 3' to 5' direction.
- Bacterial ribnuclease R [2], a 3'-5'exoribonuclease that participates in an essential cell function.
- Yeast protein SSD1 (or SRK1) which is implicated in the control of the cell cycle G1 phase.
- Yeast protein DIS3 [3], which binds to ran (GSP1) and ehances the the nucleotide-releasing activity of RCC1 on ran.
- Fission yeast protein dis3, which is implicated in mitotic control.
- Neurospora crassa cyt-4, a mitochondrial protein required for RNA 5' and 3' end processing and splicing.
- Yeast protein MSU1, which is involved in mitochondrial biogenesis.
- Synechocystis strain PCC 6803 protein zam [4], which control resistance to the carbonic anhydrase inhibitor acetazolamide.
- Caenorhabditis elegans hypothetical protein F48E8.6.
The size of these proteins range from 644 residues (rnb) to 1250 (SSD1). While their sequence is highly divergent they share a conserved domain in their C-terminal section [5]. It is possible that this domain plays a role in a putative exonuclease function that would be common to all these proteins. We have developed a signature pattern based on the core of this conserved domain.
Last update:December 2001 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Zilhao R. Camelo L. Arraiano C.M. |
| Title | DNA sequencing and expression of the gene rnb encoding Escherichia coli ribonuclease II. | |
| Source | Mol. Microbiol. 8:43-51(1993). | |
| PubMed ID | 8497196 |
| 2 | Authors | Cheng Z.-F. Zuo Y. Li Z. Rudd K.E. Deutscher M.P. |
| Title | The vacB gene required for virulence in Shigella flexneri and Escherichia coli encodes the exoribonuclease RNase R. | |
| Source | J. Biol. Chem. 273:14077-14080(1998). | |
| PubMed ID | 9603904 |
| 3 | Authors | Noguchi E. Hayashi N. Azuma Y. Seki T. Nakamura M. Nakashima N. Yanagida M. He X. Mueller U. Sazer S. Nishimoto T. |
| Title | Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. | |
| Source | EMBO J. 15:5595-5605(1996). | |
| PubMed ID | 8896453 |
| 4 | Authors | Beuf L. Bedu S. Cami B. Joset F. |
| Title | A protein is involved in accessibility of the inhibitor acetazolamide to the carbonic anhydrase(s) in the cyanobacterium Synechocystis PCC 6803. | |
| Source | Plant Mol. Biol. 27:779-788(1995). | |
| PubMed ID | 7727754 |
| 5 | Authors | Mian I.S. |
| Title | Comparative sequence analysis of ribonucleases HII, III, II PH and D. | |
| Source | Nucleic Acids Res. 25:3187-3195(1997). | |
| PubMed ID | 9241229 |
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