We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00904Ribonuclease II family signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00904
Description
On the basis of sequence similarities, the following bacterial and eukaryotic proteins seem to form a family:
- Escherichia coli and related bacteria ribonuclease II (EC 3.1.13.1) (RNase II) (gene rnb) [1]. RNase II is an exonuclease involved in mRNA decay. It degrades mRNA by hydrolyzing single-stranded polyribonucleotides processively in the 3' to 5' direction.
- Bacterial ribnuclease R [2], a 3'-5'exoribonuclease that participates in an essential cell function.
- Yeast protein SSD1 (or SRK1) which is implicated in the control of the cell cycle G1 phase.
- Yeast protein DIS3 [3], which binds to ran (GSP1) and ehances the the nucleotide-releasing activity of RCC1 on ran.
- Fission yeast protein dis3, which is implicated in mitotic control.
- Neurospora crassa cyt-4, a mitochondrial protein required for RNA 5' and 3' end processing and splicing.
- Yeast protein MSU1, which is involved in mitochondrial biogenesis.
- Synechocystis strain PCC 6803 protein zam [4], which control resistance to the carbonic anhydrase inhibitor acetazolamide.
- Caenorhabditis elegans hypothetical protein F48E8.6.
The size of these proteins range from 644 residues (rnb) to 1250 (SSD1). While their sequence is highly divergent they share a conserved domain in their C-terminal section [5]. It is possible that this domain plays a role in a putative exonuclease function that would be common to all these proteins. We have developed a signature pattern based on the core of this conserved domain.
Last update:December 2001 / Pattern and text revised.
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References
| 1 | Authors | Zilhao R. Camelo L. Arraiano C.M. |
| Title | DNA sequencing and expression of the gene rnb encoding Escherichia coli ribonuclease II. | |
| Source | Mol. Microbiol. 8:43-51(1993). | |
| PubMed ID | 8497196 |
| 2 | Authors | Cheng Z.-F. Zuo Y. Li Z. Rudd K.E. Deutscher M.P. |
| Title | The vacB gene required for virulence in Shigella flexneri and Escherichia coli encodes the exoribonuclease RNase R. | |
| Source | J. Biol. Chem. 273:14077-14080(1998). | |
| PubMed ID | 9603904 |
| 3 | Authors | Noguchi E. Hayashi N. Azuma Y. Seki T. Nakamura M. Nakashima N. Yanagida M. He X. Mueller U. Sazer S. Nishimoto T. |
| Title | Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. | |
| Source | EMBO J. 15:5595-5605(1996). | |
| PubMed ID | 8896453 |
| 4 | Authors | Beuf L. Bedu S. Cami B. Joset F. |
| Title | A protein is involved in accessibility of the inhibitor acetazolamide to the carbonic anhydrase(s) in the cyanobacterium Synechocystis PCC 6803. | |
| Source | Plant Mol. Biol. 27:779-788(1995). | |
| PubMed ID | 7727754 |
| 5 | Authors | Mian I.S. |
| Title | Comparative sequence analysis of ribonucleases HII, III, II PH and D. | |
| Source | Nucleic Acids Res. 25:3187-3195(1997). | |
| PubMed ID | 9241229 |
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