PROSITE documentation PDOC01022
TsaA-like domain signature and profile


The following uncharacterized proteins have been shown [1] to be evolutionary related and to contain a tsaA-like domain:

  • Escherichia coli hypothetical protein yaeB and HI0510, the corresponding Haemophilus influenzae protein.
  • Agrobacterium tumefaciens Ti plasmid protein virR.
  • Pseudomonas aeruginosa protein rcsF.
  • Archaeoglobus fulgidus hypothetical protein AF0241.
  • Archaeoglobus fulgidus hypothetical protein AF0433.
  • Methanococcus jannaschii hypothetical protein MJ1583.
  • Methanobacterium thermoautotrophicum hypothetical protein MTH1797.

These are proteins of from 120 to 240 amino-acid residues (with the exception of AF0433 which is 366 residues long). Although found predominantly in prokaryotes the tsaA-like domain is also found in plants, Drosophila and mammals (human, rat, mouse and others):

  • Mammalian Nef-associated protein 1 (NAP1).

The structure of the tsaA-like domain contains a six-stranded anti-parallel β-barrel (see <PDB:2NV4>). The tsaA-like domain may bind S-adenosylmethionine (AdoMet) and possesses a methyltransferase activity [2].

As a signature pattern we selected a conserved region in the central part of the domain. We also developed a profile that covers the entire tsaA-like domain.

Last update:

February 2013 / Text revised; profile added.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

TSAA_2, PS51668; TsaA-like domain profile  (MATRIX)

TSAA_1, PS01318; TsaA-like domain signature  (PATTERN)


1AuthorsBairoch A.
SourceUnpublished observations (1998).

2AuthorsForouhar F. Kuzin A. Seetharaman J. Lee I. Zhou W. Abashidze M. Chen Y. Yong W. Janjua H. Fang Y. Wang D. Cunningham K. Xiao R. Acton T.B. Pichersky E. Klessig D.F. Porter C.W. Montelione G.T. Tong L.
TitleFunctional insights from structural genomics.
SourceJ. Struct. Funct. Genomics 8:37-44(2007).
PubMed ID17588214

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