PROSITE documentation PDOC50837
NACHT-NTPase domain profile


The NACHT domain is a 300 to 400 residue predicted nucleoside triphosphatase (NTPase) domain, which is found in animal, fungal and bacterial proteins. The NACHT domain has been named after NAIP, CIITA, HET-E and TP1. It is found in association with other domains, such as the CARD domain (see <PDOC50209>), the DAPIN domain (see <PDOC50824>), the HEAT repeat (see <PDOC50077>), the WD repeat (see <PDOC00574>), the leucine-rich repeat (LRR) or the BIR repeat (see <PDOC00987) [1].

The NACHT domain consists of seven distinct conserved motifs, including the ATP/GTPase specific P-loop (see <PDOC00017>), the Mg(2+)-binding site (Walker A and B motifs, respectively) and five more specific motifs. The unique features of the NACHT domain include the prevalence of 'tiny' residues (glycine, alanine or serine) directly C-terminal of the Mg(2+)-coordinating aspartate in the Walker B motif, in place of a second acidic residue prevalent in other NTPases. A second acidic residue is typically found in the NACHT-containing proteins two positions downstream. Furthermore, the distal motif VII contains a conserved pattern of polar, aromatic and hydrophobic residues that is not seen in any other NTPase family [1].

Some proteins known to contain a NACHT domain are listed below:

  • Mammalian neuronal apoptosis inhibitor protein (NAIP).
  • Mammalian MHC class II transcription activator (CIITA).
  • Mammalian telomerase-associated protein (TP1).
  • Human CARD4 (NOD1), a pro-apoptotic protein, which activate NF-kappaB.
  • Streptomyces coelicolor predicted NTPases.
  • Synechocystis sp predicted NTPase.
  • Podosporina anserina incompatibility locus protein (HET-E).

The profile we developed covers the entire NACHT domain.

Last update:

January 2002 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

NACHT, PS50837; NACHT-NTPase domain profile  (MATRIX)


1AuthorsKoonin E.V. Aravind L.
TitleThe NACHT family - a new group of predicted NTPases implicated in apoptosis and MHC transcription activation.
SourceTrends Biochem. Sci. 25:223-224(2000).
PubMed ID10782090

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