PROSITE documentation PDOC50903
Rubredoxin-like domain profile


The rubredoxin-like domain is a 45- to 55-residue domain containing one iron atom tetrahedrally coordinated to four cysteinyl residues (Fe(S-Cys)4 center). It is involved in electron transfer processes [1,2].

The most conserved feature of the rubredoxin-like domain is the cluster of four cysteines involved in iron binding (Cys-x-x-Cys-x(n)-Cys-x-x-Cys) [2]. Structure analyses of several rubredoxin-like domains have shown that they are folded into a short three-stranded antiparallel β-sheet and a number of loops (see <PDB:1RDG>) [3,4,5,6].

Proteins known to contain a rubredoxin-like domain are listed below:

  • Rubredoxin (Rd), a small electron-transfer prokaryotic protein (see <PDOC00179>).
  • Rubrerythrin (Rr), a non-heme protein isolated from anaerobic sulfate- reducing bacteria.
  • Flavorubredoxin, a bacterial protein involved in elctron transfer processes.
  • Nigerythrin, a prokaryotic protein of unknown function.

The profile we developed covers the entire rubredoxin-like domain.

Last update:

March 2003 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

RUBREDOXIN_LIKE, PS50903; Rubredoxin-like domain profile  (MATRIX)


1Authorsvan Beeumen J.J. van Driessche G. Liu M.-Y. LeGall J.
SourceJ. Biol. Chem. 266:20645-20653(1991).

2AuthorsPrickril B.C. Kurtz D.M. Jr. LeGall J. Voordouw G.
TitleCloning and sequencing of the gene for rubrerythrin from Desulfovibrio vulgaris (Hildenborough).
SourceBiochemistry 30:11118-11123(1991).
PubMed ID1932032

3AuthorsFrey M. Sieker L. Payan F. Haser R. Bruschi M. Pepe G. LeGall J.
TitleRubredoxin from Desulfovibrio gigas. A molecular model of the oxidized form at 1.4 A resolution.
SourceJ. Mol. Biol. 197:525-541(1987).
PubMed ID3441010

4AuthorsDay M.W. Hsu B.T. Joshua-Tor L. Park J.-B. Zhou Z.H. Adams M.W.W. Rees D.C.
TitleX-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus.
SourceProtein Sci. 1:1494-1507(1992).
PubMed ID1303768

5AuthorsSieker L.C. Stenkamp R.E. LeGall J.
TitleRubredoxin in crystalline state.
SourceMethods Enzymol. 243:203-216(1994).
PubMed ID7830611

6AuthorsdeMare F. Kurtz D.M. Jr. Nordlund P.
TitleThe structure of Desulfovibrio vulgaris rubrerythrin reveals a unique combination of rubredoxin-like FeS4 and ferritin-like diiron domains.
SourceNat. Struct. Biol. 3:539-546(1996).
PubMed ID8646540

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