PROSITE documentation PDOC50969FCP1 homology domain profile
Yeast FCP1 is an essential protein serine phosphatase (EC 3.1.3.16) that dephosphorylates the C-terminal domain (CTD) of RNA polymerase II. FCP1 orthologs are present in all known eukaryote proteomes. The N-terminal domain of FCP1 corresponds to the catalytic unit of the phosphatase and has been refered to as the FCP1 homology domain. The FCP1 homology domain, which is a ~180-residue module, is also found in many other proteins of unknown function. It contains a DxDx(T/V) motif preceded by four hydrophobic residues characteristic of a large family of metal-dependent phosphohydrolases and phosphotransferases. The first aspartate residue is likely to participate in catalysis, whereas the second could have a role in substrate recognition [1,2,3,4].
The profile we developed covers the entire FCP1 homology domain.
Last update:March 2004 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Archambault J. Chambers R.S. Kobor M.S. Ho Y. Cartier M. Bolotin D. Andrews B. Kane C.M. Greenblatt J. |
| Title | An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 94:14300-14305(1997). | |
| PubMed ID | 9405607 |
| 2 | Authors | Kobor M.S. Archambault J. Lester W. Holstege F.C.P. Gileadi O. Jansma D.B. Jennings E.G. Kouyoumdjian F. Davidson A.R. Young R.A. Greenblatt J. |
| Title | An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae. | |
| Source | Mol. Cell 4:55-62(1999). | |
| PubMed ID | 10445027 |
| 3 | Authors | Hausmann S. Shuman S. |
| Title | Defining the active site of Schizosaccharomyces pombe C-terminal domain phosphatase Fcp1. | |
| Source | J. Biol. Chem. 278:13627-13632(2003). | |
| PubMed ID | 12556522 | |
| DOI | 10.1074/jbc.M213191200 |
| 4 | Authors | Cho H. Kim T.-K. Mancebo H. Lane W.S. Flores O. Reinberg D. |
| Title | A protein phosphatase functions to recycle RNA polymerase II. | |
| Source | Genes Dev. 13:1540-1552(1999). | |
| PubMed ID | 10385623 |
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