PROSITE documentation PDOC51049
KASH domain profile


The KASH (for Klarsicht/ANC-1/Syne-1 homology) or KLS domain is a highly hydrophobic nuclear envelope localization domain of ~60 amino acids comprising an ~20-amino-acid transmembrane region and a 30-35-residue C-terminal region that lies between the inner and the outer nuclear membranes. The KASH domain is found in association with other domains, such as spectrin repeats and CH (see <PDOC50021>), at the C-terminus of proteins tethered to the nuclear membrane in diverse cell types [1,2,3,4,5].

Some proteins known to contain a KASH domain are listed below:

  • Caenorhabditis elegans nuclear anchorage protein 1 (ANC-1).
  • Drosophila Klarsicht (Klar), a protein associated with nuclei and required for a subset of nuclear migrations.
  • Drosophila MSP-300.
  • Vetebrate nesprin-1 and -2 (also known as Syne-1 and -2). They are associated with emerin and lamin A at the nuclear envelope of muscle cells and other cell types.

The profile we developed covers the entire KASH domain.

Last update:

December 2004 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

KASH, PS51049; KASH domain profile  (MATRIX)


1AuthorsMosley-Bishop K.L. Li Q. Patterson L. Fischer J.A.
SourceCurr. Biol. 9:1211-1220(1999).

2AuthorsApel E.D. Lewis R.M. Grady R.M. Sanes J.R.
TitleSyne-1, a dystrophin- and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction.
SourceJ. Biol. Chem. 275:31986-31995(2000).
PubMed ID10878022

3AuthorsStarr D.A. Han M.
TitleRole of ANC-1 in tethering nuclei to the actin cytoskeleton.
SourceScience 298:406-409(2002).
PubMed ID12169658

4AuthorsZhang Q. Ragnauth C. Greener M.J. Shanahan C.M. Roberts R.G.
TitleThe nesprins are giant actin-binding proteins, orthologous to Drosophila melanogaster muscle protein MSP-300.
SourceGenomics 80:473-481(2002).
PubMed ID12408964

5AuthorsFischer J.A. Acosta S. Kenny A. Cater C. Robinson C. Hook J.
TitleDrosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye.
SourceGenetics 168:1385-1393(2004).
PubMed ID15579692

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


View entry in original PROSITE document format
View entry in raw text format (no links)