PROSITE documentation PDOC51049KASH domain profile
The KASH (for Klarsicht/ANC-1/Syne-1 homology) or KLS domain is a highly hydrophobic nuclear envelope localization domain of ~60 amino acids comprising an ~20-amino-acid transmembrane region and a 30-35-residue C-terminal region that lies between the inner and the outer nuclear membranes. The KASH domain is found in association with other domains, such as spectrin repeats and CH (see <PDOC50021>), at the C-terminus of proteins tethered to the nuclear membrane in diverse cell types [1,2,3,4,5].
Some proteins known to contain a KASH domain are listed below:
- Caenorhabditis elegans nuclear anchorage protein 1 (ANC-1).
- Drosophila Klarsicht (Klar), a protein associated with nuclei and required for a subset of nuclear migrations.
- Drosophila MSP-300.
- Vetebrate nesprin-1 and -2 (also known as Syne-1 and -2). They are associated with emerin and lamin A at the nuclear envelope of muscle cells and other cell types.
The profile we developed covers the entire KASH domain.
Last update:December 2004 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Mosley-Bishop K.L. Li Q. Patterson L. Fischer J.A. |
| Source | Curr. Biol. 9:1211-1220(1999). |
| 2 | Authors | Apel E.D. Lewis R.M. Grady R.M. Sanes J.R. |
| Title | Syne-1, a dystrophin- and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction. | |
| Source | J. Biol. Chem. 275:31986-31995(2000). | |
| PubMed ID | 10878022 | |
| DOI | 10.1074/jbc.M004775200 |
| 3 | Authors | Starr D.A. Han M. |
| Title | Role of ANC-1 in tethering nuclei to the actin cytoskeleton. | |
| Source | Science 298:406-409(2002). | |
| PubMed ID | 12169658 | |
| DOI | 10.1126/science.1075119 |
| 4 | Authors | Zhang Q. Ragnauth C. Greener M.J. Shanahan C.M. Roberts R.G. |
| Title | The nesprins are giant actin-binding proteins, orthologous to Drosophila melanogaster muscle protein MSP-300. | |
| Source | Genomics 80:473-481(2002). | |
| PubMed ID | 12408964 |
| 5 | Authors | Fischer J.A. Acosta S. Kenny A. Cater C. Robinson C. Hook J. |
| Title | Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye. | |
| Source | Genetics 168:1385-1393(2004). | |
| PubMed ID | 15579692 | |
| DOI | 10.1534/genetics.104.028662 |
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