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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC51071
RpiR-type HTH domain profile


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PURL: https://purl.expasy.org/prosite/documentation/PDOC51071

Description

The rpiR-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 75-80 amino acids present in prokaryotic regulators of transcription. The domain is named after Escherichia coli rpiR, also known as alsR, a repressor of the rpiB gene and the als operon, which encode proteins involved in sugar metabolism. The DNA-binding rpiR-type HTH domain occurs usually in the N-terminal part; the C-terminal part can contain a phosphosugar-binding SIS domain. Most rpiR-type HTH regulators are involved in sugar metabolism as transcription repressors, but some are activators [1,2,3].

Some proteins known to contain a rpiR-type HTH domain:

  • Escherichia coli rpiR, a transcription repressor of ripB, a ribose-phosphate isomerase, and of the als operon, involved in transport and metabolism of allose.
  • Pseudomonas aeruginosa hexR, a transcription repressor of the hex regulon, involved in sugar metabolism.
  • Bacillus subtilis glvR, a transcription activator of the glv operon, involved in transport and metabolism of maltose [3].

The profile we developed spans the entire rpiR-type HTH DNA-binding domain.

Last update:

January 2005 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

HTH_RPIR, PS51071; RpiR-type HTH domain profile  (MATRIX)


References

1AuthorsSorensen K.I. Hove-Jensen B.
TitleRibose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression.
SourceJ. Bacteriol. 178:1003-1011(1996).
PubMed ID8576032

2AuthorsKim C. Song S. Park C.
TitleThe D-allose operon of Escherichia coli K-12.
SourceJ. Bacteriol. 179:7631-7637(1997).
PubMed ID9401019

3AuthorsYamamoto H. Serizawa M. Thompson J. Sekiguchi J.
TitleRegulation of the glv operon in Bacillus subtilis: YfiA (GlvR) is a positive regulator of the operon that is repressed through CcpA and cre.
SourceJ. Bacteriol. 183:5110-5121(2001).
PubMed ID11489864



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